P2X receptors in the rat duodenal villus
| Autor: | Geoffrey Burnstock, Michelle Bardini, Ute Gröschel-Stewart, T. Robson |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Histology Duodenum Immunocytochemistry Crypt Molecular Sequence Data Biology digestive system Muscle Smooth Vascular Pathology and Forensic Medicine Rats Sprague-Dawley Epitopes Intestinal mucosa Antibody Specificity Internal medicine medicine Extracellular Animals Amino Acid Sequence Intestinal Mucosa Receptor Microvilli Receptors Purinergic P2 digestive oral and skin physiology Mucin Purinergic receptor Intestinal villus Muscle Smooth Cell Biology Cell biology Rats Endocrinology medicine.anatomical_structure Immunoglobulin G Female |
| Zdroj: | Cell and tissue research. 297(1) |
| ISSN: | 0302-766X |
| Popis: | Immunohistochemical techniques were performed on freshly frozen sections of the duodenum of the rat using specific polyclonal antibodies to unique peptide sequences of P2X1-7 receptors. Of the antibodies to the seven known P2X receptor subtypes that mediate extracellular signalling by nucleotides, three reacted with discrete structures in the duodenal villus of the rat. Anti-P2X1 reacted with the capillary plexus in the intestinal villus, which did not extend to the crypt region, suggesting that nucleotides may be involved in the uptake and transport of metabolites. Anti-P2X5 immunostained the membranes of the narrow "stem" of villus goblet cells, where the nucleus and cell organelles reside, possibly influencing synthesis and release of mucins. P2X7 receptor immunoreactivity was only seen in the membranes of enterocytes and goblet cells at the tip of the villus, where cells are exfoliated into the lumen, consistent with earlier findings that P2X7 is involved in apoptotic events. Thus, in complex structures such as the intestinal villus, purinoceptors appear to participate in several and diverse signalling functions. |
| Databáze: | OpenAIRE |
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