Identification and characterization of jasmonic acid- and linolenic acid-mediated transcriptional regulation of secondary laticifer differentiation in Hevea brasiliensis
| Autor: | Loh, Swee Cheng, Othman, Ahmad Sofiman, Veera Singham, G. |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant molecular biology Latex Plant physiology lcsh:Medicine Cyclopentanes Biology Genes Plant 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Organophosphorus Compounds Gene Expression Regulation Plant Transcription (biology) Plant development Transcriptional regulation Gene family MYB Oxylipins lcsh:Science Gene Plant Proteins Multidisciplinary Jasmonic acid lcsh:R alpha-Linolenic Acid biology.organism_classification Molecular biology 030104 developmental biology chemistry Laticifer Hevea lcsh:Q Hevea brasiliensis Transcription Factors 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-50800-1 |
| Popis: | Hevea brasiliensis remains the primary crop commercially exploited to obtain latex, which is produced from the articulated secondary laticifer. Here, we described the transcriptional events related to jasmonic acid (JA)- and linolenic acid (LA)-induced secondary laticifer differentiation (SLD) in H. brasiliensis clone RRIM 600 based on RNA-seq approach. Histochemical approach proved that JA- and LA-treated samples resulted in SLD in H. brasiliensis when compared to ethephon and untreated control. RNA-seq data resulted in 86,614 unigenes, of which 2,664 genes were differentially expressed in JA and LA-induced secondary laticifer harvested from H. brasiliensis bark samples. Among these, 450 genes were unique to JA and LA as they were not differentially expressed in ethephon-treated samples compared with the untreated samples. Most transcription factors from the JA- and LA-specific dataset were classified under MYB, APETALA2/ethylene response factor (AP2/ERF), and basic-helix-loop-helix (bHLH) gene families that were involved in tissue developmental pathways, and we proposed that Bel5-GA2 oxidase 1-KNOTTED-like homeobox complex are likely involved in JA- and LA-induced SLD in H. brasiliensis. We also discovered alternative spliced transcripts, putative novel transcripts, and cis-natural antisense transcript pairs related to SLD event. This study has advanced understanding on the transcriptional regulatory network of SLD in H. brasiliensis. |
| Databáze: | OpenAIRE |
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