Hypoxic Preconditioning Confers Long-Term Reduction of Brain Injury and Improvement of Neurological Ability in Immature Rats
| Autor: | Henrik Hagberg, Malin Gustavsson, Michelle F. Anderson, Carina Mallard |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Nervous system
Time Factors Central nervous system Morris water navigation task Brain damage Cerebro Brain Ischemia Rats Sprague-Dawley Lesion Animals Medicine Hypoxia Ischemic Preconditioning Maze Learning business.industry Hypoxia (medical) Rats Oxygen medicine.anatomical_structure Animals Newborn Brain Injuries Anesthesia Hypoxia-Ischemia Brain Pediatrics Perinatology and Child Health Brain Damage Chronic medicine.symptom Forelimb business |
| Zdroj: | Pediatric Research. 57:305-309 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/01.pdr.0000151122.58665.70 |
| Popis: | Exposure to preconditioning (PC) hypoxia 24 h before a severe hypoxic-ischemic (HI) insult reduces development of injury in the immature brain. Several protective regimens have proved effective in the short-term but not in the long-term perspective. The aim of the present study, therefore, was to evaluate the PC effect on long-term morphologic and neurologic outcome in the developing brain. Six-day-old rats were subjected to hypoxia (36 degrees C, 8.0% O2; PC/HI group) and sham controls to normoxia (36 degrees C; HI group) for 3 h. Twenty-four hours later, all rats were exposed to cerebral HI produced by unilateral carotid artery occlusion combined with 1 h, 15 min of hypoxia (36 degrees C, 7.7% O2). A cylinder test was used to evaluate forelimb asymmetry to determine sensorimotor function at 4, 6, and 8 wk of age. Spatial/cognitive ability was assessed by Morris water maze trials at 7 wk of recovery. Neuropathologic analysis was performed 8 wk after insult. Brain damage was reduced (p0.0001) in PC/HI (45.0+/-11.1 mm3) in comparison with HI (159.3+/-12.2 mm3) rats. A bias for using the ipsilateral forelimb in wall movements was observed in the cylinder test in HI compared with PC/HI rats at 4 (p0.001), 6 (p0.01), and 8 (p0.0001) wk of age. Results of the Morris water maze test revealed differences (p0.0001) in average path length between groups on the third and fourth day of trials. Hypoxic PC before HI reduced brain injury by 72% at 8 wk after the insult and provided long-term improvement of sensorimotor and spatial/cognitive functions. |
| Databáze: | OpenAIRE |
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