Comparative analysis of differentially expressed sequence tags of sweet orange and mandarin infected with Xylella fastidiosa
| Autor: | Juliano F. Ravasi, Carlos Norberto Fischer, Helvécio D. Coletta-Filho, E. F. Carlos, Magnólia de Araújo Campos, Marcos Antonio Machado, Juliana E.C. Teixeira, Alessandra A. de Souza, Maria Luisa P.N. Targon, Marco Aurélio Takita |
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| Přispěvatelé: | Agência Paulista de Tecnologia dos Agronegócios (APTA), Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Universidade Federal de Lavras (UFLA), Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2007 |
| Předmět: |
Differential display
Expressed sequence tag Chlorosis lcsh:QH426-470 biology ESTs citrus variegated chlorosis CitEST food and beverages Orange (colour) Biotic stress biology.organism_classification lcsh:Genetics phytopathogen biotic stress Botany Genetics Cultivar Xylella fastidiosa Molecular Biology Citrus × sinensis |
| Zdroj: | Genetics and Molecular Biology v.30 n.3 suppl.0 2007 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG ResearcherID Genetics and Molecular Biology, Vol 30, Iss 3, Pp 965-971 (2007) SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genetics and Molecular Biology, Volume: 30, Issue: 3 Supplement, Pages: 965-971, Published: 2007 |
| ISSN: | 1415-4757 |
| DOI: | 10.1590/s1415-47572007000500024 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:04:59Z No. of bitstreams: 1 S1415-47572007000500024.pdf: 111101 bytes, checksum: bbf06b30276bcde974c4179aa2e2fe66 (MD5) Made available in DSpace on 2013-08-22T19:04:59Z (GMT). No. of bitstreams: 1 S1415-47572007000500024.pdf: 111101 bytes, checksum: bbf06b30276bcde974c4179aa2e2fe66 (MD5) Previous issue date: 2007-01-01 Made available in DSpace on 2013-09-30T20:08:34Z (GMT). No. of bitstreams: 2 S1415-47572007000500024.pdf: 111101 bytes, checksum: bbf06b30276bcde974c4179aa2e2fe66 (MD5) S1415-47572007000500024.pdf.txt: 30205 bytes, checksum: 6ea5fe1357dc6c9e00d761beca3701fc (MD5) Previous issue date: 2007-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:17:30Z No. of bitstreams: 2 S1415-47572007000500024.pdf: 111101 bytes, checksum: bbf06b30276bcde974c4179aa2e2fe66 (MD5) S1415-47572007000500024.pdf.txt: 30205 bytes, checksum: 6ea5fe1357dc6c9e00d761beca3701fc (MD5) Made available in DSpace on 2014-05-20T15:17:30Z (GMT). No. of bitstreams: 2 S1415-47572007000500024.pdf: 111101 bytes, checksum: bbf06b30276bcde974c4179aa2e2fe66 (MD5) S1415-47572007000500024.pdf.txt: 30205 bytes, checksum: 6ea5fe1357dc6c9e00d761beca3701fc (MD5) Previous issue date: 2007-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. on the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly susceptible cultivar to X. fastidiosa) and mandarin Ponkan (tolerant) artificially infected with the bacterium. Using an in silico differential display, 172 genes were found to be significantly differentially expressed in such conditions. Sweet orange presented an increase in expression of photosynthesis related genes that could reveal a strategy to counterbalance a possible lower photosynthetic activity resulting from early effects of the bacterial colonization in affected plants. on the other hand, mandarin showed an active multi-component defense response against the bacterium similar to the non-host resistance pattern. Instituto Agronômico de Campinas Centro APTA Citros Sylvio Moreira Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais Universidade Federal de Lavras (UFLA) Departamento de Biologia Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Genética e Fitopatologia Universidade Estadual Paulista Instituto de Geociências e Ciências Exatas Departamento de Estatística, Matemática Aplicada e Computação Universidade Estadual Paulista Instituto de Geociências e Ciências Exatas Departamento de Estatística, Matemática Aplicada e Computação |
| Databáze: | OpenAIRE |
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