Cholesterol modulates PAF-stimulated Ca2+-mobilization in monocytic U937 cells
| Autor: | Martin Aepfelbacher, I. Lux, Peter C. Weber, N. Hrboticky |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Biophysics chemistry.chemical_element Stimulation Calcium Biochemistry Monocytes Cell Line chemistry.chemical_compound Adenosine Triphosphate Internal medicine medicine Humans Platelet Activating Factor Molecular Biology U937 cell Platelet-activating factor Cholesterol Monocyte Lipoproteins LDL Kinetics Cytosol medicine.anatomical_structure Endocrinology Receptors LDL chemistry Cell culture SRS-A lipids (amino acids peptides and proteins) Lipoproteins HDL |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1074:125-129 |
| ISSN: | 0304-4165 |
| Popis: | We investigated the effect of cellular cholesterol content on platelet activating factor (PAF)-stimulated Ca2+ mobilization in the human monocytic cell line U937. When cholesterol auxotroph U937 cells were depleted of cellular cholesterol by a 48-h incubation in delipidated medium, a 40% reduction in the PAF (100 nM)-stimulated increase in cytosolic Ca2+ concentration was seen. Ca2+ mobilization following stimulation with LTD4 (10 nM) or ATP (10 microM) was not affected. Addition of LDL (100 micrograms/ml, 24 h) to the delipidated medium completely recovered cellular cholesterol content and PAF-induced Ca2+ mobilization. These two LDL effects had very similar time- and dose-dependences. Partial recoveries of PAF-induced Ca2+ mobilization, seen after addition of pure cholesterol dissolved in ethanol (30 micrograms/ml, 24 h) or acetyl-LDL (100 micrograms/ml, 24 h), were associated with partial recoveries of cellular cholesterol content. Our results indicate that cellular cholesterol influences PAF-stimulated events in monocytic cells. |
| Databáze: | OpenAIRE |
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