High-level expression of recombinant human nerve growth factor beta in milk of nontransgenic rabbits
| Autor: | Bo Xiao, Zhongliang Jiang, Kun Li, Bo Feng, Zeng Sheng Han, Qingwang Li, Rui Zhao, Jian Li, Xu Bo Zhi, Jian Hong Hu, Dawei Gao |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Mammary gland Gene Expression Bioengineering Chick Embryo Biology medicine.disease_cause PC12 Cells Applied Microbiology and Biotechnology Adenoviridae law.invention Mammary Glands Animal Dorsal root ganglion Transduction Genetic law Ganglia Spinal Internal medicine Nerve Growth Factor medicine Animals Humans Lagomorpha Embryo Biological activity biology.organism_classification Molecular biology Recombinant Proteins Rats Milk Nerve growth factor medicine.anatomical_structure Endocrinology Recombinant DNA Female Rabbits Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 105:327-334 |
| ISSN: | 1389-1723 |
| DOI: | 10.1263/jbb.105.327 |
| Popis: | The technology for the large-scale production of therapeutic recombinant proteins remains a challenge in the biopharmaceutical industry. In this study, we reported a nontransgenic approach to producing a large quantity of human nerve growth factor beta (hNGF-beta) in rabbit milk by employing a recombinant adenoviral expression system. After directly instilling hNGF-beta recombinant adenoviruses into rabbit mammary glands, a polypeptide with a molecular weight of 13.2 kDa was detected in rabbit milk. The maximal expression level of hNGF-beta reached 346 mug/ml. The biological activity of recombinant hNGF-beta was confirmed using PC12 cells and cultures of dorsal root ganglion neurons from chicken embryos. Our data suggest that instilling recombinant adenovirus directly into the mammary gland of mammals is an efficient approach to producing a large quantity of hNGF-beta. |
| Databáze: | OpenAIRE |
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