Isolation of ovine β-lactoglobulin genetic variants by chromatofocusing: Heterogeneity of β-lactoglobulin A

Autor: Mercedes Ramos, M. D. Fernández-Esplá, G. López-Gálvez
Přispěvatelé: Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Rok vydání: 1993
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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ISSN: 1612-1112
0009-5893
Popis: Chromatofocusing was applied to the isolation of ovine β-Lactoglubulin (β-Lg) genetic variants. Ovine β-Lg A and B, which are known to differ only by a single amino-acid substitution, were separated. Moreover, one component differing from A variant in isoelectric point and relative molecular mass has been isolated in samples containing ovine β-Lg A. Although elution of β-Lgs occurred far from their “normal” isoelectric points, chromatofocusing is a powerful technique effective for both analytical and preparative scale.
Research work was supported by the Comisión Interministerial de Ciencia y Tecnología (Project ALI 91-0784).
Databáze: OpenAIRE
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