Correlation between antibody affinity and serum bactericidal activity in infants
| Autor: | Martha L. Lepow, Seth Hetherington |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Blood Bactericidal Activity
Diphtheria Toxoid animal diseases Radioimmunoassay Enzyme-Linked Immunosorbent Assay medicine.disease_cause Haemophilus influenzae Microbiology Conjugate vaccine medicine Immunology and Allergy Humans Haemophilus Vaccines biology Toxoid Infant Antibodies Bacterial Bacterial vaccine Infectious Diseases Bacterial Vaccines biology.protein Regression Analysis Immunization Antibody Conjugate |
| Zdroj: | The Journal of infectious diseases. 165(4) |
| ISSN: | 0022-1899 |
| Popis: | Nearly one-half of infants immunized with Haemophilus influenzae b capsular polysaccharide (polyribosylribitol phosphate; PRP)-protein conjugate produce low-affinity antibody. To test the hypothesis that antibody affinity is linked to biologic function, sera were obtained before and 1 month after immunization of 18-month-old infants with PRP-diphtheria toxoid conjugate vaccine. Correlation was attempted of anti-PRP affinity, concentrations of anti-PRP, and anti-outer membrane proteins and of immunoglobulin isotype with bactericidal activity. Nine subjects produced anti-PRP of low affinity (K less than 10(4) l/mol), and 11 had higher affinity antibodies (average K, 2.8 x 10(4) l/mol). By multiple regression analysis, antibody affinity was the only variable significantly related to the bactericidal activity of serum after immunization with the conjugate vaccine (r = .71; P = .04). Thus, serum anti-PRP from a substantial proportion of infants appeared functionally deficient in association with low-affinity antibody. |
| Databáze: | OpenAIRE |
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