Reperfusion-induced leukocyte adhesion and vascular protein leakage in normal and hypercholesterolemic rats
| Autor: | J. Tolley, Norman R. Harris, D C Anderson, D. N. Granger, Iwao Kurose, Masayuki Miyasaka, L. W. Argenbright |
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| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Physiology medicine.drug_class Hypercholesterolemia Myocardial Reperfusion Injury CD18 Monoclonal antibody Microcirculation Capillary Permeability Rats Sprague-Dawley Platelet Adhesiveness Cell Movement Ischemia Reference Values Physiology (medical) Internal medicine Cell Adhesion Leukocytes medicine Animals Splanchnic Circulation Cell adhesion Serum Albumin business.industry Albumin Extravasation Rats Endothelial stem cell Endocrinology Immunology Cardiology and Cardiovascular Medicine business Intracellular |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 273:H854-H860 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1997.273.2.h854 |
| Popis: | The objective of this study was to define the influence of hypercholesterolemia on ischemia-reperfusion (I/R)-induced leukocyte-endothelial cell adhesion and albumin leakage in rat mesenteric venules. The microvascular alterations normally elicited by I/R (leukocyte adherence and emigration, albumin leakage, and platelet aggregation) were more pronounced in hypercholesterolemic rats (compared with control rats). Monoclonal antibodies against the adhesion glycoproteins CD11/CD18 and intercellular adhesion molecule-1 attenuated the I/R-induced leukocyte adherence and emigration and albumin leakage. Leukocyte adherence, but not albumin leakage, was diminished in animals pretreated with a P-selectin-specific antibody. Platelet aggregation was reduced by antibodies directed against either P-selectin, CD18, or intercellular adhesion molecule-1, as well as a GPIIb-IIIa antagonist. These results indicate that the enhanced reperfusion-induced albumin leakage in hypercholesterolemic rats is dependent on leukocyte-endothelial cell adhesion. Furthermore, P-selectin- and CD11/CD18-dependent heterotypic and GPIIb-IIIa-mediated homotypic platelet aggregation appear to influence the extravasation of both leukocytes and albumin in postischemic venules of hypercholesterolemic rats. |
| Databáze: | OpenAIRE |
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