Effects of two multi-step self-etch primer/adhesives on apoptosis in human gingival fibroblastsin vitro
| Autor: | Özer Aylin Gürpınar, Arzu Beklen, Mehmet Ali Onur, Yrjö T. Konttinen, Zafer C. Cehreli, Mika Hukkanen |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Materials science
0206 medical engineering Gingiva Biomedical Engineering Apoptosis Biocompatible Materials 02 engineering and technology Biomaterials Dental Materials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Humans Staurosporine Propidium iodide Fragmentation (cell biology) 030304 developmental biology 0303 health sciences Cell growth Acridine orange 030206 dentistry Fibroblasts 021001 nanoscience & nanotechnology 020601 biomedical engineering Molecular biology In vitro Staining Biochemistry chemistry Tissue Adhesives 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. :435-440 |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.30558 |
| Popis: | Various in vitro studies have shown induction of apoptosis by monomers incorporated to dental restorative materials and adhesive resins, while information regarding the effect of monomer combinations as commercially available products on apoptosis is limited. The aim of this study was to investigate the effects of two multi-step self-etch primer/adhesive systems on apoptosis of cultured primary human gingival fibroblasts. Cells were treated up to 48 h with Clearfil SE Bond (Kuraray, Japan) and FL Bond (Shofu, Japan) at 1:1000 v:v ratio to determine cell proliferation, using 0.02 mM staurosporine as positive control. Apoptosis was assessed using propidium iodide/acridine orange (PI/AO) staining, compared to nontreated controls. When compared to FL Bond, exposure of gingival fibroblasts to Clearfil SE Primer and Clearfil SE Bond resulted in a higher degree of cell proliferation. PI/AO staining revealed typical morphological features of apoptosis in FL Bond and Staurosporine groups, while some cells cultured in the presence of primer and adhesive components of Clearfil SE Bond showed nuclear fragmentation, indicative of early apoptosis. Our results indicate that apoptotic potential of the multi-step self-etch adhesives were material-dependent within the 48 h test period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text