Use of a white light supercontinuum laser for confocal interference-reflection microscopy
| Autor: | Liang-da Chiu, William Amos, Stefanie Reichelt, L. Su |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Histology
Materials science Microscope Light Confocal Physics::Optics Fluorescent Antibody Technique Confocal scanning microscopy Pathology and Forensic Medicine law.invention Mice Optics Two-photon excitation microscopy law Animals Confocal laser scanning microscopy Microscopy Interference interference reflection microscopy Microscopy Confocal business.industry Lasers Scanning confocal electron microscopy Original Articles 3T3 Cells Fibroblasts Laser Supercontinuum Light sheet fluorescence microscopy supercontinuum laser Optoelectronics business |
| Zdroj: | Journal of Microscopy |
| ISSN: | 1365-2818 0022-2720 |
| Popis: | Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for the multiple monochromatic lasers normally used for the excitation of fluorescence. This light source is now available coupled to commercial confocal fluorescence microscopes. We have evaluated a supercontinuum laser as a source for a different purpose: confocal interferometric imaging of living cells and artificial models by interference reflection. We used light in the range 460–700 nm where this source provides a reasonably flat spectrum, and obtained images free from fringe artefacts caused by the longer coherence length of conventional lasers. We have also obtained images of cytoskeletal detail that is difficult to see with a monochromatic laser. |
| Databáze: | OpenAIRE |
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