Bifidobacterium jacchi sp. nov., isolated from the faeces of a baby common marmoset (Callithrix jacchus)
| Autor: | Maria Satti, Monica Marianna Modesto, Samanta Michelini, Koichi Watanabe, Barbara Sgorbati, Paola Mattarelli, Masanori Arita, Cesare Cammà, Kaihei Oki, Claudio Patavino, Piero Sciavilla |
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| Přispěvatelé: | Modesto, Monica, Watanabe, Koichi, Arita, Masanori, Satti, Maria, Oki, Kaihei, Sciavilla, Piero, Patavino, Claudio, Cammà, Cesare, Michelini, Samanta, Sgorbati, Barbara, Mattarelli, Paola |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial Biology Callithrix jacchu 010603 evolutionary biology 01 natural sciences Microbiology common marmoset 03 medical and health sciences Feces Phylogenetics Bacterial Typing Technique RNA Ribosomal 16S Genotype Animals Gene Ecology Evolution Behavior and Systematics Phylogeny Bifidobacterium Genetics Base Composition Phylogenetic tree Animal Strain (biology) Fatty Acids Nucleic Acid Hybridization Callithrix General Medicine Sequence Analysis DNA biology.organism_classification rpoB 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology new specie Genes Bacterial Fece Bifidobacterium jacchi Fatty Acid |
| Popis: | A novel Bifidobacterium strain, MRM 9.3T, was isolated from a faecal sample of a baby common marmoset (Callithrixjacchus). Cells were Gram-stain-positive, non-motile, non-sporulating, non-haemolytic, facultatively anaerobic and fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA genes as well as multilocus sequences (representing hsp60, rpoB, clpC, dnaJ and dnaG genes) and the core genomes revealed that strain MRM 9.3T exhibited phylogenetic relatedness to Bifidobacterium myosotis DSM 100196T. Comparative analysis of 16S rRNA gene sequences confirmed the phylogenetic results showing the highest gene sequence identity with strain B.ifidobacterium myosotis DSM 100196T (95.6 %). The average nucleotide identity, amino acid average identity and in silico DNA–DNA hybridization values between MRM 9.3T and DSM 100196T were 79.9, 72.1 and 28.5 %, respectively. Phenotypic and genotypic features clearly showed that the strain MRM 9.3T represents a novel species, for which the name Bifidobacterium jacchi sp. nov. is proposed. The type strain is MRM 9.3T (=DSM 103362T =JCM 31788T). |
| Databáze: | OpenAIRE |
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