Broad early immune response of porcine epithelial jejunal IPI-2I cells to Entamoeba histolytica

Autor: Sandrine Melo, François Meurens, Aurore Grave, Henri Salmon, Fabienne Girard-Misguich, Nancy Guillén
Přispěvatelé: Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Biologie Cellulaire du Parasitisme, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), This work was supported in part by the transversal research program INRA-Pasteur (PTR-291, 'Development of an immunopathologic swine model for intestin
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Chemokine
MESH: Dysentery
Amebic
MESH: Swine Diseases
Phagocyte
Swine
NEUTROPHIL
EPITHELIAL CELLS
ENTAMOEBA HISTOLYTICA
0302 clinical medicine
MESH: Up-Regulation
MESH: Animals
Intestinal Mucosa
MESH: Swine
Swine Diseases
MESH: Cytokines
0303 health sciences
biology
Entamoeba histolytica
Acquired immune system
3. Good health
Up-Regulation
medicine.anatomical_structure
Jejunum
MESH: Intestinal Mucosa
Dysentery
Amebic
Cytokines
[SDV.IMM]Life Sciences [q-bio]/Immunology
MESH: Immunity
Innate
medicine.symptom
Immunology
Inflammation
Microbiology
Cell Line
03 medical and health sciences
Immune system
INNATE RESPONSE
parasitic diseases
medicine
Animals
Molecular Biology
030304 developmental biology
PIG
Innate immune system
MESH: Entamoeba histolytica
biology.organism_classification
Immunity
Innate
MESH: Cell Line
CCL20
MESH: Jejunum
biology.protein
030215 immunology
Zdroj: Molecular Immunology
Molecular Immunology, Elsevier, 2009, 46 (5), pp.927-936
Molecular Immunology, 2009, 46 (5), pp.927-936. ⟨10.1016/j.molimm.2008.09.036⟩
ISSN: 0161-5890
DOI: 10.1016/j.molimm.2008.09.036⟩
Popis: International audience; Amoebiasis caused by Entamoeba histolytica triggers an acute inflammatory response at early stages of intestinal infection. The patho-physiological study of intestinal amoebiasis requires the development of powerful animal models. Swine provide robust model for human diseases and they could be used to study intestinal amoebiasis. Here, we introduce an in vitro model of swine intestinal epithelial cell (IPI-21) co-cultured with E. histolytica. Intestinal epithelial cells (IECs) have crucial roles in sensing pathogens and initiating innate immune response, which qualitatively influence adaptive immune response against them. The contact between the two cells induces marked macroscopic lesions of IEC monolayer and striking alteration of the IPI-21 cell phenotype including blebbing, such as loss of attachment before to be phagocyte by the trophozoite. Increase in Lactate Dehydrogenase (LDH) levels in the culture supernatant of IECs was observed when ameba is present and could reflect the cellular cytotoxicity exerted by the parasite. Using quantitative real-time PCR, we identified the up-regulation of cytokines/chemokines implicated in neutrophil chemoattraction and inflammation, such as CCL2, CCL20, CXCL2, CXCL3, GM-CSF, IL1 alpha, IL6 and IL8, in response to the parasite that can further regulate the immunoregulatory functions of the immune cells of the host. The study points a cardinal role of these pro-inflammatory compounds as central mediators in the interaction IECs/ameba and suggests mechanisms by which they coordinate intestinal immune response. This will focus future efforts on delineating the molecular and cellular mechanisms of other cell partners by the way of in vivo infection of swine. (C) 2008 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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