Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol
| Autor: | Agnieszka Zimna, Agnieszka Malcher, Łukasz Kubaszewski, Natalia Rozwadowska, Magdalena Nowaczyk, Wojciech Barczak, Błażej Rubiś, Maciej Kurpisz, Wojciech Łabędź |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Physiology Clinical Biochemistry Population SOD2 heart failure RM1-950 medicine.disease_cause Biochemistry Article 03 medical and health sciences 0302 clinical medicine Gene expression medicine α-phenyl-N-tert-butyl nitrone oxidative stress Progenitor cell education Molecular Biology education.field_of_study Chemistry Myogenesis apoptosis Cell Biology Hypoxia (medical) Cell biology 030104 developmental biology Apoptosis post-infarction heart Therapeutics. Pharmacology medicine.symptom skeletal muscle derived stem cells 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Antioxidants Volume 10 Issue 6 Antioxidants, Vol 10, Iss 959, p 959 (2021) |
| ISSN: | 2076-3921 |
| Popis: | The aim of the study was to modify human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) and demonstrate the optimal cell preparation protocol for application in post-infarction hearts. We used conditioned SkMDS/PC culture medium with α-phenyl-N-tert-butyl nitrone (PBN). SkMDS/PCs were cultured under hypoxic conditions and the results were compared to the standard ones. We observed a significant increase of CD-56 positive phenotypic marker the ability to form functional myotubes, increase in the proportion of young cells in cell primary suspensions, and a decrease in the percentage of apoptotic cells among PBN-conditioned cells in normoxia an hypoxia. We also observed significantly higher levels of SOD3 expression maintained expression of SOD1, SOD2, and CAT a higher level of BCL2 gene expression and a rather significant decrease in Hsp70 gene expression in PBN-conditioned SkMDS/PCs compared to the WT population under hypoxic conditions. In addition, significant increase of myogenic genes expression was observed after PBN addition to culture medium, compared to WT population under hypoxia. Interestingly, PBN addition significantly increased the lengths of telomeres under hypoxia. Based on the data obtained, we can postulate that PBN conditioning of human SkMDS/PCs could be a promising step in improving myogenic cell preparation protocol for pro-regenerative treatment of post-infarction hearts. |
| Databáze: | OpenAIRE |
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