Site-dependent differential KIT and PDGFRA expression in gastric and intestinal gastrointestinal stromal tumors
| Autor: | Bastian Gunawan, Florian Haller, Thomas Armbrust, Claus Langer, Detlef Doenecke, László Füzesi, S. Schwager, Hans-Jürgen Schulten, Anja von Heydebreck, Nicole Happel |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Stromal cell Receptor Platelet-Derived Growth Factor alpha Gastrointestinal Stromal Tumors Gene Expression PDGFRA Biology medicine.disease_cause Disease-Free Survival Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms Germany Gene expression Intestinal Neoplasms medicine Biomarkers Tumor Humans neoplasms 030304 developmental biology Aged Regulation of gene expression Aged 80 and over 0303 health sciences Mutation GiST Stomach Middle Aged digestive system diseases Gene Expression Regulation Neoplastic Survival Rate Proto-Oncogene Proteins c-kit medicine.anatomical_structure Real-time polymerase chain reaction 030220 oncology & carcinogenesis Cancer research Female Stromal Cells |
| Zdroj: | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 20(10) |
| ISSN: | 0893-3952 |
| Popis: | In gastrointestinal stromal tumors (GISTs), mutually exclusive gain-of-function mutations of KIT and PDGFRA are associated with different mutation-dependent clinical behavior. Taking into account the well-known different clinical behavior of GISTs from the stomach or the intestine, the aim of the current study is to evaluate the mutation- and site-dependent effects on mRNA and protein expression of KIT and PDGFRA in a large series of primary GISTs. Fresh-frozen tissue of 53 primary GISTs from gastric (75%) or intestinal (25%) sites were analyzed for mutation of KIT or PDGFRA using direct sequencing. Furthermore, KIT and PDGFRA mRNA and protein expression were determined using quantitative RT-PCR and quantitative densitometric evaluation of Western blot data. Each tumor either had a mutation of KIT (79%) or PDGFRA (21%). All GISTs with PDGFRA mutation were from gastric sites. Mutation-dependently, GISTs with KIT mutation had a significantly higher expression of KIT and at the same time a significantly lower expression of PDGFRA compared to GISTs with PDGFRA mutation. Site-dependently, gastric GISTs had a significantly higher expression of PDGFRA and a significantly lower expression of KIT compared to intestinal GISTs. Additionally, even if the KIT-mutated GISTs alone were considered, a significantly higher expression of PDGFRA could be observed in gastric than in intestinal tumors. We also found a significant correlation between a higher protein expression of PDGFRA and longer disease-free survival. The correlation of gastric site and PDGFRA mutation with higher PDGFRA expression and longer disease-free survival suggests different regulatory roles of KIT and PDGFRA gene expression on the control of cell proliferation, and, thereby on clinical behavior. The higher PDGFRA expression in gastric GISTs possibly contributes to the well-known site-dependent clinical behavior. |
| Databáze: | OpenAIRE |
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