Using PATIMDB to Create Bacterial Transposon Insertion Mutant Libraries
| Autor: | Frederick M. Ausubel, Jonathan M. Urbach, Nicole T. Liberati, Gang Wu, Jacinto Villanueva, Tao Wei, Daniel Grenfell-Lee |
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| Rok vydání: | 2009 |
| Předmět: |
Genetics
Transposable element Bacteria Sequence analysis business.industry Mutant Process (computing) Computational Biology Biology computer.software_genre Software package Pipeline (software) Article Mutagenesis Insertional Software Operating system Genomic library business Molecular Biology computer Gene Library |
| Zdroj: | Current Protocols in Molecular Biology |
| ISSN: | 1934-3647 1934-3639 |
| DOI: | 10.1002/0471142727.mb1907s86 |
| Popis: | PATIMDB is a software package for facilitating the generation of transposon mutant insertion libraries. The software has two main functions: process tracking and automated sequence analysis. The process tracking function specifically includes recording the status and fates of multiwell plates and samples in various stages of library construction. Automated sequence analysis refers specifically to the pipeline of sequence analysis starting with ABI files from a sequencing facility and ending with insertion location identifications. The protocols in this unit describe installation and use of PATIMDB software. |
| Databáze: | OpenAIRE |
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