Early Tissue Patterning Recreated by Mouse Embryonic Fibroblasts in a Three-Dimensional Environment
| Autor: | Salvador Borrós, Elsa Genové, María Del Mar Olmos, Carlos E. Semino, Teresa Fernández Muiños, Lluis Quintana |
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| Rok vydání: | 2009 |
| Předmět: |
Fetal Proteins
Brachyury Time Factors Cellular differentiation Cell Culture Techniques Biomedical Engineering Morphogenesis Core Binding Factor Alpha 1 Subunit Bioengineering Biology Models Biological Biochemistry Regenerative medicine Article Dictyostelium discoideum Biomaterials Mice Tissue engineering Cell Movement Animals Cells Cultured Cell Proliferation Tissue Scaffolds Regeneration (biology) Gene Expression Regulation Developmental Cell Differentiation SOX9 Transcription Factor Fibroblasts Embryo Mammalian biology.organism_classification Embryonic stem cell Nanostructures Cell biology Mice Inbred C57BL T-Box Domain Proteins |
| Zdroj: | Tissue Engineering Part A. 15:45-54 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2007.0296 |
| Popis: | Cellular self-organization studies have been mainly focused on models such as Volvox, the slime mold Dictyostelium discoideum, and animal (metazoan) embryos. Moreover, animal tissues undergoing regeneration also exhibit properties of embryonic systems such as the self-organization process that rebuilds tissue complexity and function. We speculated that the recreation in vitro of the biological, biophysical, and biomechanical conditions similar to those of a regenerative milieu could elicit the intrinsic capacity of differentiated cells to proceed to the development of a tissue-like structure. Here we show that, when primary mouse embryonic fibroblasts are cultured in a soft nanofiber scaffold, they establish a cellular network that causes an organized cell contraction, proliferation, and migration that ends in the formation of a symmetrically bilateral structure with a distinct central axis. A subset of mesodermal genes (brachyury, Sox9, Runx2) is upregulated during this morphogenetic process. The expression of brachyury was localized first at the central axis, extending then to both sides of the structure. The spontaneous formation of cartilage-like tissue mainly at the paraxial zone followed expression of Sox9 and Runx2. Because cellular self-organization is an intrinsic property of the tissues undergoing development, this model could lead to new ways to consider tissue engineering and regenerative medicine. |
| Databáze: | OpenAIRE |
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