Comparison of Three Serological Methods for the Epidemiological Investigation of TBE in Dogs
| Autor: | Maja Haut, Sandra Riederer, Martin Pfeffer, Gerhard Dobler, Philipp Girl |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Veterinary medicine Specific test 040301 veterinary sciences 030231 tropical medicine TBEV seroprevalence dog ELISA IIFA micro-NT Microbiology Serology 0403 veterinary science 03 medical and health sciences Emerging pathogen 0302 clinical medicine Virology Epidemiology medicine Seroprevalence lcsh:QH301-705.5 business.industry Communication Incidence (epidemiology) Endemic area 04 agricultural and veterinary sciences medicine.disease lcsh:Biology (General) ddc:540 business Encephalitis |
| Zdroj: | Microorganisms, Vol 9, Iss 399, p 399 (2021) Microorganisms |
| ISSN: | 2076-2607 |
| Popis: | Tick-borne encephalitis (TBE) virus is an emerging pathogen that causes severe infections in humans. Infection risk areas are mostly defined based on the incidence of human cases, a method which does not work well in areas with sporadic TBE cases. Thus, sentinel animals may help to better estimate the existing risk. Serological tests should be thoroughly evaluated for this purpose. Here, we tested three test formats to assess the use of dogs as sentinel animals. A total of 208 dog sera from a known endemic area in Southern Germany were tested in an All-Species-ELISA and indirect immunofluorescence assays (IIFA), according to the manufacturer’s instructions. Sensitivity and specificity for both were determined in comparison to the micro-neutralization test (NT) results. Of all 208 samples, 22.1% tested positive in the micro-NT. A total of 18.3% of the samples showed characteristic fluorescence in the IIFA and were, thus, judged positive. In comparison to the micro-NT, a sensitivity of 78.3% and a specificity of 98.8% was obtained. In the ELISA, 19.2% of samples tested positive, with a sensitivity of 84.8% and a specificity of 99.4%. The ELISA is a highly specific test for TBE-antibody detection in dogs and should be well suited for acute diagnostics. However, due to deficits in sensitivity, it cannot replace the NT, at least for epidemiological studies. With even lower specificity and sensitivity, the same applies to IIFA. |
| Databáze: | OpenAIRE |
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