Supplemental nitrite increases choroidal neovascularization in mice
| Autor: | Michael E. Boulton, Karina C. Ricart, Xiaoping Qi, Rakesh P. Patel, Khandaker Ahtesham Ahmed |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
medicine.medical_specialty genetic structures Physiology Clinical Biochemistry Biochemistry Article Nitric oxide Lesion Macular Degeneration Mice chemistry.chemical_compound Nitrate Internal medicine Wet age-related macular degeneration medicine Animals Nitrite Sodium nitrite Nitrites Nitrates business.industry Histology Choroidal Neovascularization eye diseases Mice Inbred C57BL Endocrinology Choroidal neovascularization chemistry Female sense organs medicine.symptom business |
| Zdroj: | Nitric Oxide |
| ISSN: | 1089-8603 |
| Popis: | Low doses of nitrite, close to physiological levels, increase blood flow in normal and ischemic tissues through a nitric oxide (NO) dependent mechanism. Given that nitrite therapy and dietary supplementation with vegetables high in nitrate (e.g. beets) are gaining popularity we decided to determine if low doses of nitrite impact the development of choroidal neovascularization (CNV), a key feature of wet age related macular degeneration (AMD). Sodium nitrite (at 50 mg/L, 150 mg/L, and 300 mg/L), nitrate (1 g/L) or water alone were provided in the drinking water of C57BL/6J mice aged 2 or 12 months. Mice were allowed to drink ad libitum for 1 week at which time laser-induced choroidal neovascularization (L-CNV) was induced. The mice continued to drink the supplemented water ad libitum for a further 14 days at which point optical coherence tomography (OCT) was performed to determine the volume of the CNV lesion. Blood was drawn to determine nitrite and nitrate levels and eyes taken for histology. CNV volume was 2.86 × 10(7) μm(3) (±0.4×10(7)) in young mice on water alone but CNV volume more than doubled to >6.9 × 10(7) μm(3) (±0.8×10(7)) in mice receiving 300 mg/L nitrite or 7.34 × 10(7) μm(3) (±1.4×10(7)) in 1 g/L nitrate (p |
| Databáze: | OpenAIRE |
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