Effects of ovariectomy on the inputs from the medial nucleus of the amygdala to the ventromedial nucleus of the hypothalamus in young adult rats

Autor: Denise Ribeiro Gobbo, Lais da Silva Pereira, Susana Isabel Sá, Jozélia G. Ferreira, José Anchieta de Castro Horta-Junior, Jackson C. Bittencourt
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Univ Porto
Rok vydání: 2020
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1872-7972
Popis: Made available in DSpace on 2021-06-25T15:00:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-16 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) National Funds through the FCT - Fundacao para a Ciencia e a Tecnologia within CINTESIS During puberty, sexual hormones induce crucial changes in neural circuit organization, leading to significant sexual dimorphism in adult behaviours. The ventrolateral division of the ventromedial nucleus of the hypothalamus (VMHyl) is the major neural site controlling the receptive component of female sexual behaviour, which is dependent on ovarian hormones. The inputs to the VMHyl, originating from the medial nucleus of the amygdala (MeA), transmit essential information to trigger such behaviour. In this study, we investigated the projection pattern of the MeA to the VMHyl in ovariectomized rats at early puberty. Six-week-old SpragueDawley rats were ovariectomized (OVX) and, upon reaching 90 days of age, were subjected to iontophoretic injections of the neuronal anterograde tracer Phaseolus vulgaris leucoagglutinin into the MeA. Projections from the MeA to the VMHyl and to other structures included in the neural circuit responsible for female sexual behaviour were analysed in the Control and OVX groups. The results of the semi-quantitative analysis showed that penpubertal ovariectomy reduced the density of intra-amygdalar fibres. The stereological estimates, however, failed to find changes in the organization of the terminal fields of nerve fibres from the MeA to the VMHyl in the adult. The present data show that ovariectomized rats during the peripubertal phase did not undergo significant changes in MeA fibres reaching the VMHyl; however, they suggest a possible effect of ovariectomy on MeA connectivity under amygdalar subnuclei. Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Lab Neuroanat Quim, Sao Paulo, SP, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Anat, Botucatu, SP, Brazil Univ Sao Paulo, Inst Psicol Nucleo Neurociencias & Comportamento, Sao Paulo, SP, Brazil Univ Porto, Fac Med, Dept Biomed, Unit Anat, Porto, Portugal Univ Porto, Fac Med, Ctr Hlth Technol & Serv Res, CINTESIS, Porto, Portugal Univ Estadual Paulista, Inst Biociencias, Dept Anat, Botucatu, SP, Brazil FAPESP: 2010/52068-0 FAPESP: 2016/02224-1 FAPESP: 2015/05990-4 CAPES: 848/15 CNPq: 426378/2016-4 National Funds through the FCT - Fundacao para a Ciencia e a Tecnologia within CINTESIS: UID/IC/4255/2013
Databáze: OpenAIRE
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