Degradation of chondroitin 4-sulphate by tri-fluoroacetolysis: isolation of oligosaccharides from the carbohydrate-protein linkage region
| Autor: | Alf Gunnarsson, Lennart Rodén, Sigfrid Svensson |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Chromatography
Gas Stereochemistry Ion chromatography Oligosaccharides Xylose Biochemistry Methylation Gas Chromatography-Mass Spectrometry Mass Spectrometry Analytical Chemistry Glycosaminoglycan chemistry.chemical_compound Carbohydrate Conformation Chondroitin Tetrasaccharide Animals Trifluoroacetic Acid Glycoproteins Nasal Septum Organic Chemistry Chondroitin Sulfates General Medicine Carbohydrate Paper chromatography chemistry Cattle Chondroitin 4 sulfate |
| Zdroj: | Carbohydrate research. 133(1) |
| ISSN: | 0008-6215 |
| Popis: | Oligosaccharides from the linkage-region tetrasaccharide, beta-D-GlcpA-(1----3)-beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D- Xylp, of chondroitin 4-sulphate were isolated after trifluoroacetolysis. The oligosaccharides were purified by ion-exchange chromatography and paper chromatography and subjected to sugar and methylation analysis and g.l.c.-m.s. The recovery of linkage-region oligosaccharides was approximately 45% after trifluoroacetolysis, calculated according to the D-xylose present in the chondroitin 4-sulphate preparation. The following structures were identified: beta-D-Galp-(1----4)-D-Xylp, beta-D-Galp-(1----3)-D-Galp, beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D-Xylp, beta-D-GlcpA-(1----3)-beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D- Xylp. |
| Databáze: | OpenAIRE |
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