Whole cell biotransformation for reductive amination reactions
| Autor: | Elisabeth Lorenz, Volker F. Wendisch, Stephanie Klatte |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
biology
Bioengineering General Medicine Applied Microbiology and Biotechnology Reductive amination Keto Acids Cofactor Corynebacterium glutamicum Metabolic engineering Biochemistry Biotransformation Alanine Dehydrogenase Glucose dehydrogenase biology.protein Escherichia coli NADPH regeneration Isoleucine Transaminases Biotechnology Alcohol dehydrogenase Bacillus subtilis |
| DOI: | 10.4161/bioe.27151 |
| Popis: | Whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. Recently, we established an Escherichia coli whole cell biotransformation system for reductive amination by coupling a transaminase and an amino acid dehydrogenase with glucose catabolism for cofactor recycling. Transformation of 2-keto-3-methylvalerate to l-isoleucine by E. coli cells was improved by genetic engineering of glucose metabolism for improved cofactor regeneration. Here, we compare this system with different strategies for cofactor regeneration such as cascading with alcohol dehydrogenases, with alternative production hosts such as Pseudomonas species or Corynebacterium glutamicum, and with improving whole cell biotransformation systems by metabolic engineering of NADPH regeneration. |
| Databáze: | OpenAIRE |
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