A Multipotential Leukemia Cell Line (K-562) of Human Origin
| Autor: | Elena G. Bamberger, Bismarck B. Lozzio, Aurora S. Feliu, Carmen B. Lozzio |
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| Rok vydání: | 1981 |
| Předmět: |
Leukemia
Experimental Cell division Isoelectric focusing Cellular differentiation Cell Differentiation Golgi apparatus Biology medicine.disease General Biochemistry Genetics and Molecular Biology Cell Line Hematopoiesis Cell biology symbols.namesake Haematopoiesis Leukemia Biochemistry Cell culture symbols medicine Animals Humans Hemoglobin |
| Zdroj: | Experimental Biology and Medicine. 166:546-550 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-166-41106 |
| Popis: | The K-562 leukemia cell line, originally established in our laboratory, has been characterized as an early precursor of the granulocytic series with a block for differentiation. Since K-562 blasts did not differentiate when cultured for 7-8 days in liquid media or 14-16 days in agar-gel an attempt was made to stimulate their potential for spontaneous differentiation by prolonging the time in culture. Inducers of differentiation were not added to the cultures and the cells were studied when they reached the steady state rather than during exponential growth. The cultivation of K-562 cells for 10 to 11 days in media gradually depleted of the essential nutrients needed for cell division induced their differentiation into early precursors of the monocytic, granulocytic, and erythrocytic series. Thus, the peroxidase reaction for hemoglobin demonstrates benzidine-positive material limited to the region of the Golgi apparatus. Analysis of the hemoglobin by isoelectric focusing indicated major bands in th... |
| Databáze: | OpenAIRE |
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