Phenotypic Analysis of Meltrin α (ADAM12)-Deficient Mice: Involvement of Meltrin α in Adipogenesis and Myogenesis
| Autor: | Tomohiro Kurisaki, Atsushi B. Tsuji, Akira Nagabukuro, Yoichiro Iwakura, Shigeki Higashiyama, Masahide Asano, Yo-ichi Nabeshima, Junko Sakagami, Atsuko Sehara-Fujisawa, Aki Masuda, Yoichi Matsuda, Katsuko Sudo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_treatment
ADAM12 Protein Muscle Proteins Adipose tissue Biology Mice Epidermal growth factor Mammalian Genetic Models with Minimal or Complex Phenotypes medicine Animals Regeneration Muscle Skeletal Molecular Biology Epidermal Growth Factor Myogenesis Growth factor Chromosome Mapping Gene Expression Regulation Developmental Membrane Proteins Gene targeting Skeletal muscle Embryo Cell Biology Fibroblasts Embryo Mammalian Molecular biology Mice Mutant Strains Rats Cell biology ADAM Proteins Phenotype medicine.anatomical_structure Adipose Tissue Animals Newborn Adipogenesis Intercellular Signaling Peptides and Proteins Tetradecanoylphorbol Acetate Heparin-binding EGF-like Growth Factor |
| Zdroj: | Molecular and Cellular Biology. 23:55-61 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.23.1.55-61.2003 |
| Popis: | Meltrin alpha (ADAM12) is a metalloprotease-disintegrin whose specific expression patterns during development suggest that it is involved in myogenesis and the development of other organs. To determine the roles Meltrin alpha plays in vivo, we generated Meltrin alpha-deficient mice by gene targeting. Although the number of homozygous embryos are close to the expected Mendelian ratio at embryonic days 17 to 18, ca. 30% of the null pups born die before weaning, mostly within 1 week of birth. The viable homozygous mutants appear normal and are fertile. Most of the muscles in the homozygous mutants appear normal, and regeneration in experimentally damaged skeletal muscle is unimpeded. In some Meltrin alpha-deficient pups, the interscapular brown adipose tissue is reduced, although the penetrance of this phenotype is low. Impaired formation of the neck and interscapular muscles is also seen in some homozygotes. These observations suggest Meltrin alpha may be involved in regulating adipogenesis and myogenesis through a linked developmental pathway. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a candidate substrate of Meltrin alpha, and we found that TPA (12-O-tetradecanoylphorbol-13-acetate)-induced ectodomain shedding of HB-EGF is markedly reduced in embryonic fibroblasts prepared from Meltrin alpha-deficient mice. We also report here the chromosomal locations of Meltrin alpha in the mouse and rat. |
| Databáze: | OpenAIRE |
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