Effect of Cell Density on Hormonal Secretion from Human Pituitary Adenomas in vitro
| Autor: | Leslie J. Hipkin, Alex M. Landolt, Patrick M. Foy, M. C. White, R. V. Jeffreys, Stephen L. Atkin |
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| Rok vydání: | 1998 |
| Předmět: |
Adenoma
Adult Male endocrine system medicine.medical_specialty endocrine system diseases Somatotropic cell Basophil cell Endocrinology Diabetes and Metabolism Cell Count Pituitary neoplasm Biology Prolactin cell Endocrinology Pituitary Hormones Anterior Internal medicine Tumor Cells Cultured medicine Humans Pituitary Neoplasms Secretion Aged Retrospective Studies Middle Aged medicine.disease In vitro Pediatrics Perinatology and Child Health Female hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Hormone Research in Paediatrics. 49:203-209 |
| ISSN: | 1663-2826 1663-2818 |
| DOI: | 10.1159/000023172 |
| Popis: | Cell density effects were investigated on tumorous hormonal secretion from 10 pituitary adenomas: 3 somatotrophinomas secreting GH and PRL; 7 gonadotrophinomas, 3 co-secreted both FSH and LH, all 7 secreted LH. Enzymatically dispersed tissue was plated out in 24-well plates at 5 ×105, 105, 5 ×104 and 104 cells/well in serum-free media. Media were collected weekly for 2 weeks. Results: In 3 of 3 somatotrophinomas, GH and PRL secretion was higher (p < 0.05) at both week 1 and 2 from 104 cells/well, but similar at other cell densities. In all 3 gonadotrophinomas, the FSH secretory rate was highest at 5 ×105 cells/well which fell as cell density decreased. Conversely, in 7 of 7 gonadotrophinomas the LH secretory rate was highest at 104 cells/well (p < 0.01) which fell as cell density increased. Conclusion: These data suggest that paracrine factors may modulate tumorous GH, PRL, FSH and LH secretion, and show that FSH and LH secretion vary inversely as cell density increases. |
| Databáze: | OpenAIRE |
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