The 3D-structure, kinetics and dynamics of the E. coli nitroreductase NfsA with NADP

Autor:	Scott A. White, Andrew J. Christofferson, Alastair I. Grainger, Martin A. Day, David Jarrom, Antonio E. Graziano, Peter F. Searle, Eva I. Hyde
Rok vydání:	2022
Předmět:	Niacinamide Escherichia coli Proteins Biophysics Quinones Cell Biology Nitroreductases Hydroxylamines NAD Biochemistry Anti-Bacterial Agents Hydroquinones Phosphates Kinetics Structural Biology Genetics Escherichia coli Prodrugs Molecular Biology NADP
Zdroj:	FEBS lettersReferences. 596(18)
ISSN:	1873-3468
Popis:	Nitroreductases activate nitroaromatic antibiotics and cancer prodrugs to cytotoxic hydroxylamines and reduce quinones to quinols. Using steady-state and stopped-flow kinetics, we show that the Escherichia coli nitroreductase NfsA is 20-50 fold more active with NADPH than with NADH and that product release may be rate-limiting. The crystal structure of NfsA with NADP
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f979dcdbf0eb9b669a15bb17e6bc49df https://pubmed.ncbi.nlm.nih.gov/35648111 Zobrazit plný text záznamu Plný text