In Vitro Diffusion of the Immunosuppressant Tacrolimus Through Human and Rabbit Corneas
| Autor: | David A. Meyer, Armorel D van Eyk, Pieter van der Bijl |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Diffusion Permeability Tacrolimus Cornea Andrology Species Specificity Pharmacokinetics medicine Animals Humans Pharmacology (medical) Aged Aged 80 and over Pharmacology Lagomorpha biology Chemistry Rabbit (nuclear engineering) Middle Aged biology.organism_classification eye diseases In vitro Ophthalmology medicine.anatomical_structure Permeability (electromagnetism) Models Animal Immunology Female Rabbits sense organs Ophthalmic Solutions Immunosuppressive Agents |
| Zdroj: | Journal of Ocular Pharmacology and Therapeutics. 23:146-151 |
| ISSN: | 1557-7732 1080-7683 |
| DOI: | 10.1089/jop.2006.0105 |
| Popis: | The aim of this study was to investigate the in vitro diffusion kinetics of tacrolimus (TAC) through rabbit and human corneas, and to evaluate the suitability of rabbit cornea as an in vitro permeability model for human cornea.A flow-through diffusion apparatus was used for all permeability experiments. The percentage of total (3)H-TAC diffusing across the corneas was determined over 24 h and fractions collected twice-hourly (20 degrees C; 1.5 mL/h). An F test was used for whole-curve comparisons, with a significance level of 5%.Steady-state values for TAC across both types of corneas were not reached. Although the percentage of TAC diffusing across rabbit cornea was higher than that found for human cornea up to 18 h, there were no statistically significant differences. The percentages of total TAC diffusing across human and rabbit cornea at the 24-h time points were 0.38% +/- 0.02% and 0.36% +/- 0.01%, respectively.Percentages of total TAC at the 24-h time points were approximately 4.9x and 4.1x higher than those found previously for cyclosporin A across human and rabbit corneas, respectively. Rabbit corneas are a suitable model for human corneas in in vitro permeability studies. |
| Databáze: | OpenAIRE |
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