SHARPIN promotes cell proliferation of cholangiocarcinoma and inhibits ferroptosis via p53/ SLC7A11 / GPX4 signaling
Autor: | Chong Zeng, Jie Lin, Ketao Zhang, Huohui Ou, Ke Shen, Qingbo Liu, Zibo Wei, Xinhuai Dong, Xiaokang Zeng, Liming Zeng, Weidong Wang, Jie Yao |
---|---|
Rok vydání: | 2022 |
Předmět: |
Cancer Research
Amino Acid Transport System y+ General Medicine Phospholipid Hydroperoxide Glutathione Peroxidase Cholangiocarcinoma Bile Ducts Intrahepatic Bile Duct Neoplasms Oncology Cell Line Tumor Humans Ferroptosis Tumor Suppressor Protein p53 Reactive Oxygen Species Ubiquitins Cell Proliferation Signal Transduction |
Zdroj: | Cancer Science. 113:3766-3775 |
ISSN: | 1349-7006 1347-9032 |
Popis: | SHARPIN is a tumor-associated gene involved in the growth and proliferation of many tumor types. A function of SHARPIN in cholangiocarcinoma (CCA) is so far unclear. Here, we studied the role and function of SHARPIN in CCA and revealed its relevant molecular mechanism. The expression of SHARPIN was analyzed in cholangiocarcinoma tissues from patients using immunohistochemistry, quantitative PCR, and western blot analysis. Expression of SHARPIN was suppressed/overexpressed by siRNA silencing or lentiviral overexpression vector, and the effect on cell proliferation was determined by the CCK-8 assay and flow cytometry. Accumulation of reactive oxygen species was measured with MitoTracker, and JC-1 staining showed mitochondrial fission/fusion and mitochondrial membrane potential changes as a result of the silencing or overexpression. The ferroptosis marker solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), and the antioxidant enzymes superoxide dismutase 1 (SOD-1) and SOD-2 were analyzed by western blot. The results showed that SHARPIN expression was increased in CCA tissue, and this was involved in cell proliferation. SHARPIN silencing resulted in accumulated reactive oxygen species, reduced mitochondrial fission, and a reduced mitochondrial membrane potential. Silencing of SHARPIN inhibited the ubiquitination and degradation of p53, and downregulated levels of SLC7A11, GPX4, SOD-1, and SOD-2, all of which contributed to excessive oxidative stress that leads to ferroptosis. Overexpression of SHARPIN would reverse the above process. The collected data suggest that in CCA, SHARPIN-mediated cell ferroptosis via the p53/SLC7A11/GPX4 signaling pathway is inhibited. Targeting SHARPIN might be a promising approach for the treatment of CCA. |
Databáze: | OpenAIRE |
Externí odkaz: |