Fluorescence in situ hybridization and chromosome studies after transfusion in newborns: Is a waiting period necessary?
| Autor: | Violeta Komotos, Jennifer Fynn, Lorene Schweig, Debra Rita, Raghbir Benawra, Bhagya L. Puppala, Denise B. Angst |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Genetics Chromosomes Human X Chromosomes Human Y Erythrocytes medicine.diagnostic_test Graft Survival Infant Newborn Chromosome Biology Molecular biology Waiting period Karyotyping Leukocytes medicine Humans Female Leukocyte Reduction Procedures Erythrocyte Transfusion In Situ Hybridization Genetics (clinical) Fluorescence in situ hybridization |
| Zdroj: | Genetics in Medicine. 7:54-57 |
| ISSN: | 1098-3600 |
| Popis: | Delaying chromosome studies after transfusion is common practice in many neonatal intensive care units (NICUs). Yet, no evidence exists to support this practice.To investigate the effects of filtration and irradiation on chromosome detection, and to evaluate donor chromosome interference after transfusion.Packed red blood cells (PRBCs) were evaluated by fluorescence in situ hybridization (FISH) and chromosome analyses. To evaluate donor leukocyte survival, blood was collected from female neonates who received male-donated PRBCs.Irradiated, leukodepleted blood had no Y chromosome detection by FISH. Irradiated, microaggregate filtered blood had Y chromosome detection in all samples by FISH but no metaphase growth. No donor chromosomes were detected in neonates after transfusion.Delaying chromosome or FISH analysis in transfused neonates who have received irradiated blood is unnecessary. |
| Databáze: | OpenAIRE |
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