Comparative evaluation of loop-mediated isothermal amplification (LAMP) vs qPCR for detection of Toxoplasma gondii oocysts DNA in mussels
| Autor: | Alessia Possenti, Alain Geffard, Loïc Durand, Marco Lalle, Jitender P. Dubey, Stéphanie La Carbona |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030231 tropical medicine Immunology Loop-mediated isothermal amplification Apicomplexan parasite Biology Real-Time Polymerase Chain Reaction Sensitivity and Specificity Microbiology Comparative evaluation Foodborne Diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hemolymph parasitic diseases medicine Animals Electrophoresis Agar Gel fungi Toxoplasma gondii General Medicine DNA Protozoan 030108 mycology & parasitology medicine.disease biology.organism_classification Toxoplasmosis Bivalvia Infectious Diseases chemistry Parasitology Nucleic Acid Amplification Techniques Toxoplasma DNA |
| Zdroj: | Experimental Parasitology. 208:107809 |
| ISSN: | 0014-4894 |
| Popis: | The apicomplexan parasite Toxoplasma gondii can infect humans and cause toxoplasmosis. T. gondii has been highly prioritized among the foodborne parasites regarding its global impact on public health. Human infection can occur through multiple routes, including the ingestion of raw or undercooked food contaminated with T. gondii oocysts, such as fresh produce and bivalves. As filter-feeders, bivalves can accumulate and concentrate contaminants, including protozoan (oo)cysts. Although detection of T. gondii in different bivalves by molecular techniques (PCR and qPCR) has been achieved, routine application is currently limited by lack of sensitivity or equipment costs. Here, we describe the assessment of a loop-mediated isothermal amplification (LAMP)-based assay to detect T. gondii oocysts in spiked mussels. Detection limit was down to 5 oocysts/g in tissue and 5 oocyst/ml in hemolymph, and, under the experimental conditions tested, LAMP was found to provide a promising alternative to qPCR. |
| Databáze: | OpenAIRE |
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