Energy-rich phosphates in slow and fast human skeletal muscle
| Autor: | John S. Leigh, Krista Vandenborne, K De Meirleir, Glenn A. Walter, R. Staron, L. Ploutz-Snyder, A. Fry, G. A. Dudley |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Magnetic Resonance Spectroscopy Adolescent Physiology Phosphates Phosphocreatine chemistry.chemical_compound Myosin medicine Humans Muscle Skeletal Soleus muscle Physical Education and Training biology Succinate dehydrogenase Reproducibility of Results Skeletal muscle Phosphorus Cell Biology Nuclear magnetic resonance spectroscopy musculoskeletal system Phosphate Succinate Dehydrogenase Muscle Fibers Slow-Twitch medicine.anatomical_structure Biochemistry chemistry Muscle Fibers Fast-Twitch biology.protein Biophysics Energy Metabolism Myofibril Sports |
| Zdroj: | American Journal of Physiology-Cell Physiology. 268:C869-C876 |
| ISSN: | 1522-1563 0363-6143 |
| Popis: | We investigated the relationship between energy-rich phosphate content and muscle fiber-type composition in human skeletal muscle using a combination of 31P-nuclear magnetic resonance spectroscopy (NMR), histochemical, and biochemical analyses of muscle biopsies. Localized 31P spectra were collected simultaneously from the predominantly slow-twitch soleus muscle and the mixed (fast-twitch and slow-twitch) medial and lateral gastrocnemius muscles, using B1-insensitive Hadamard Spectroscopic Imaging. Biopsy samples were taken from the soleus and lateral gastrocnemius muscles before NMR investigation and analyzed for fiber type composition and succinic dehydrogenase (SDH) activity. Fiber-type composition was determined based both on myofibrillar actomyosin ATPase activity combined with cross-sectional area and on myosin heavy-chain composition. Localized spectroscopy demonstrated a significantly (P < 0.001) higher P(i)/phosphocreatine ratio in the soleus muscle (0.15 +/- 0.01) compared with the medial (0.12 +/- 0.01) and lateral (0.10 +/- 0.0) gastrocnemius. However, in vitro analysis of muscle biopsies showed only a moderate relationship between the basal phosphate content and myofibrillar actomyosin ATPase-based fiber-type composition and SDH activity, respectively. |
| Databáze: | OpenAIRE |
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