Natural evolution of hepatitis C virus infection in hemodialysis Tunisian patients and CTLA-4 SNP's
| Autor: | Imen Sfar, Mouna Makhlouf, Taieb Ben Abdallah, Khaled Ayed, Leila Ksiaa Cheikhrouhou, S. Jendoubi-Ayed, H. Aouadi, Yousr Lakhoua-Gorgi |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Time Factors viruses Hepacivirus medicine.medical_treatment medicine.disease_cause Gene Frequency Risk Factors Odds Ratio CTLA-4 Antigen Cross Infection biology Gastroenterology virus diseases hemic and immune systems General Medicine Hepatitis C Middle Aged Viral Load Prognosis Phenotype Female Hemodialysis Viral load Adult Tunisia Hepatitis C virus chemical and pharmacologic phenomena Polymorphism Single Nucleotide Renal Dialysis medicine Humans SNP Genetic Predisposition to Disease Allele frequency Aged Retrospective Studies Chi-Square Distribution Haplotype Transfusion Reaction Case Control Study biology.organism_classification medicine.disease Virology biological factors Logistic Models Haplotypes Immunology |
| Zdroj: | World Journal of Gastroenterology. 21:10150-10158 |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v21.i35.10150 |
| Popis: | To analyze the polymorphisms of CTLA-4 gene involved in the response against hepatitis C virus (HCV) infection.We recruited 500 hemodialysed patients from several hemodialysis centers, all HCV-antibody positive, spread over different regions of Tunisia, as part of a national survey in 2008 conducted in the laboratory of immunology at the Charles Nicolle hospital Tunisia, classified into two groups G1 (PCR+) and G2 (PCR-) according to the presence or absence of viral RNA. Of these patients, 307 were followed prospectively on a viral molecular level over a period from 2002 to 2008, divided into two groups based on the persistence and viral clearance. PCR-RFLP was performed for the analysis of SNPs (+49) A/G and (+6230) G/A CTLA-4 for these 500 patients and 358 healthy controls.Analysis of clinical and virological characteristics of our cohort suggests a nosocomial infection in our hemodialysed patients with transfusion history as a primary risk factor and a predominance of genotype 1b. The haplotype analysis revealed an increase of frequencies of GG (+49)/(CT60) CTLA-4 in the entire patients group compared to controls (P = 0.0036 and OR = 1.42; 95%CI: 1.12-1.79, respectively). This haplotype is therefore associated with susceptibility to HCV infection.Our study suggests a possible role of CTLA-4 polymorphisms in the outcome of HCV infection in the Tunisian hemodialysed population. |
| Databáze: | OpenAIRE |
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