Complex coordinated extracellular metabolism: Acid phosphatases activate diluted human leukocyte proteins to generate energy flow as NADPH from purine nucleotide ribose
| Autor: | James E. Cox, John B. Hibbs, Zdenek Vavrin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Purine GSH reduced glutathione Ribose Clinical Biochemistry Glutathione reductase Biochemistry Pentose Phosphate Pathway AUC area under the curve chemistry.chemical_compound NET neutrophil extracellular trap PNP purine nucleoside phosphorylase Leukocyte and human prostate acid phosphatases Leukocytes LC–MS liquid chromatography–mass spectrometry Nucleotide Purine Nucleotides lcsh:QH301-705.5 chemistry.chemical_classification lcsh:R5-920 Glutathione Disulfide GSSG oxidized glutathione FGT female genital tract 3. Good health XOR xanthine oxidoreductase OB-PPP oxidative branch pentose phosphate pathway lcsh:Medicine (General) Oxidation-Reduction Transaldolase Research Paper GC–MS gas chromatography-mass spectrometry PAP human prostate acid phosphatase u units Acid Phosphatase Pentose phosphate pathway Biology Pi inorganic phosphate 03 medical and health sciences Humans Extracellular metabolism PNCP purine nucleotide catabolic pathway NO nitric oxide 030102 biochemistry & molecular biology Organic Chemistry Metabolism Human leukocytes 030104 developmental biology Enzyme chemistry lcsh:Biology (General) Redox regulation Energy Metabolism TLS tumor lysis syndrome NADP NOB-PPP non-oxidative branch pentose phosphate pathway |
| Zdroj: | Redox Biology, Vol 8, Iss C, Pp 271-284 (2016) Redox Biology |
| ISSN: | 2213-2317 |
| Popis: | Complex metabolism is thought to occur exclusively in the crowded intracellular environment. Here we report that diluted enzymes from lysed human leukocytes produce extracellular energy. Our findings involve two pathways: the purine nucleotide catabolic pathway and the pentose phosphate pathway, which function together to generate energy as NADPH. Glucose6P fuel for NADPH production is generated from structural ribose of purine ribonucleoside monophosphates, ADP, and ADP-ribose. NADPH drives glutathione reductase to reduce an oxidized glutathione disulfide-glutathione redox couple. Acid phosphatases initiate ribose5P salvage from purine ribonucleoside monophosphates, and transaldolase controls the direction of carbon chain flow through the nonoxidative branch of the pentose phosphate pathway. These metabolic control points are regulated by pH. Biologically, this energy conserving metabolism could function in perturbed extracellular spaces. Graphical abstract fx1 Highlights • Extracellular metabolism occurs in dilute human leukocyte cytosolic protein solution. • Extracellular metabolism is activated by acid phosphatases. • Ribose5P salvage from purine nucleotides and its metabolism produces NADPH. • Extracellular NADPH generating metabolism influences the redox environment. • Extracellular metabolism could be a component of inflammation and wound healing. |
| Databáze: | OpenAIRE |
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