The Epidermis-Specific Extracellular BODYGUARD Controls Cuticle Development and Morphogenesis inArabidopsis
| Autor: | Alexander Yephremov, Rochus Franke, Nadia Efremova, Sascha Bär, Christiane Nawrath, Jean-Pierre Métraux, Derry Voisin, Lukas Schreiber, Sergey Kurdyukov, Heinz Saedler, Andrea Faust |
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| Rok vydání: | 2006 |
| Předmět: |
Cutinase
Protein Folding Hydrolases Cuticle Molecular Sequence Data Arabidopsis Morphogenesis Plant Science Cutin Plant Epidermis Protein Carbonylation Cell wall Gene Expression Regulation Plant Arabidopsis thaliana Amino Acid Sequence RNA Messenger Cloning Molecular Research Articles integumentary system biology Epidermis (botany) Arabidopsis Proteins Genetic Complementation Test Gene Expression Regulation Developmental Cell Biology Plants Genetically Modified biology.organism_classification Lipids Plant Leaves Protein Transport Phenotype Biochemistry Organ Specificity Waxes DNA Transposable Elements Sequence Alignment |
| Zdroj: | The Plant Cell. 18:321-339 |
| ISSN: | 1532-298X |
| DOI: | 10.1105/tpc.105.036079 |
| Popis: | The outermost epidermal cell wall is specialized to withstand pathogens and natural stresses, and lipid-based cuticular polymers are the major barrier against incursions. The Arabidopsis thaliana mutant bodyguard (bdg), which exhibits defects characteristic of the loss of cuticle structure not attributable to a lack of typical cutin monomers, unexpectedly accumulates significantly more cell wall–bound lipids and epicuticular waxes than wild-type plants. Pleiotropic effects of the bdg mutation on growth, viability, and cell differentiation are also observed. BDG encodes a member of the α/β-hydrolase fold protein superfamily and is expressed exclusively in epidermal cells. Using Strep-tag epitope-tagged BDG for mutant complementation and immunolocalization, we show that BDG is a polarly localized protein that accumulates in the outermost cell wall in the epidermis. With regard to the appearance and structure of the cuticle, the phenotype conferred by bdg is reminiscent of that of transgenic Arabidopsis plants that express an extracellular fungal cutinase, suggesting that bdg may be incapable of completing the polymerization of carboxylic esters in the cuticular layer of the cell wall or the cuticle proper. We propose that BDG codes for an extracellular synthase responsible for the formation of cuticle. The alternative hypothesis proposes that BDG controls the proliferation/differentiation status of the epidermis via an unknown mechanism. |
| Databáze: | OpenAIRE |
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