Superior sensitivity of novel molecular imaging probe: simultaneously targeting two types of endothelial injury markers
| Autor: | Fang Xie, SouskaZandi Zandi, Dawei Sun, Alexander Schering, Shintaro Nakao, Ali Hafezi-Moghadam |
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| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Endothelium P-selectin Retinal Artery Intercellular Adhesion Molecule-1 Anti-Inflammatory Agents Inflammation Biology Biochemistry Dexamethasone Research Communications Uveitis In vivo Leukocytes Genetics medicine Humans Molecular Biology Fluorescent Dyes Choroid Cell adhesion molecule Microspheres Capillaries Scanning laser ophthalmoscopy P-Selectin Early Diagnosis medicine.anatomical_structure Microscopy Fluorescence Endothelium Vascular medicine.symptom Molecular imaging Biomarkers Biotechnology |
| Zdroj: | The FASEB Journal. 24:1532-1540 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.09-148981 |
| Popis: | The need remains great for early diagnosis of diseases. The special structure of the eye provides a unique opportunity for noninvasive light-based imaging of fundus vasculature. To detect endothelial injury at the early and reversible stage of adhesion molecule up-regulation, we generated novel imaging agents that target two distinct types of endothelial molecules, a mediator of rolling, P-selectin, and one that mediates firm adhesion, ICAM-1. Interactions of these double-conjugated fluorescent microspheres (MSs) in retinal or choroidal microvasculature were visualized in live animals by scanning laser ophthalmoscopy. The new imaging agents showed significantly higher sensitivity for detection of endothelial injury than singly conjugated MSs (rPSGL-1- or α-ICAM-1-conjugated), both in terms of rolling (P |
| Databáze: | OpenAIRE |
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