Validation of the Biofish-300 HIS Enzymatic Biosensor for the Detection of Histamine in Fishery Products
| Autor: | Sandra Salleres, James M. Hungerford, Ronald A. Benner, Irune González, Roberto González, Arrate Jaureguibeitia, Alai Arantzamendi, Sonia Maza, Christina A. Mireles DeWitt |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Calibration curve Fisheries Mackerel Biosensing Techniques 01 natural sciences Analytical Chemistry Matrix (chemical analysis) 03 medical and health sciences chemistry.chemical_compound Anchovy Fish Products Animals Environmental Chemistry Electrodes Pharmacology biology 010401 analytical chemistry Sardine food and beverages biology.organism_classification 0104 chemical sciences Fishery 030104 developmental biology chemistry Aromatic-L-Amino-Acid Decarboxylases Calibration Tuna Agronomy and Crop Science Biosensor Histamine Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 99:1338-1355 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | The Biofish-300 HIS method is a simple, reliable, and specific enzymatic biosensor for the detection of histamine. This technology is highly specific and selective and allows quantification of histamine in fishery products (fresh/frozen and processed) in a short time frame (2–3 min). Histamine in raw tuna, raw mackerel, raw sardine, raw anchovy, boiled tuna, canned tuna in water, canned tuna in oil, canned mackerel in tomato sauce, canned pickled sardine, and canned salted anchovy was analyzed using a water-based extract. Matrix-specific assay procedures and calibration curves were used to enable analyses to be carried out across multiple sample types. The performance of this assay was examined using samples that were naturally contaminated (reference materials and interlaboratory studies) and spiked with histamine. All data were judged against previously established acceptance criteria. Performance measures were evaluated for linearity, selectivity, matrix, lot consistency, and robustness. Results produced in all performance measures, except robustness, were within acceptable ranges. Out-of-range robustness results reflected deviation in sample volume compared to the standard assay procedures. Positive interferences from the presence of agmatine were shown. |
| Databáze: | OpenAIRE |
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