Chemopreventive Effect of Curcuma longa Linn on Liver Pathology in HBx Transgenic Mice
| Autor: | Yeon-Weol Lee, Chong-Kwan Cho, Hyung-Bae Moon, Hwa-Seung Yoo, Hye-Lin Ha, Dae-Yeul Yu, Jung-Sun Kim |
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| Rok vydání: | 2010 |
| Předmět: |
Blood Glucose
Cyclin-Dependent Kinase Inhibitor p21 Male Carcinoma Hepatocellular Cirrhosis Transgene Gene Expression Mice Transgenic Intra-Abdominal Fat medicine.disease_cause Chemoprevention Mice Curcuma Liver Neoplasms Experimental Cyclin D1 hemic and lymphatic diseases medicine Animals Viral Regulatory and Accessory Proteins Phosphorylation Cell Proliferation Hepatitis B virus biology Plant Extracts Body Weight Organ Size medicine.disease digestive system diseases Liver Regeneration Proliferating cell nuclear antigen Mice Inbred C57BL HBx Liver Complementary and alternative medicine Oncology Hepatocellular carcinoma Immunology Hepatocytes Mice Inbred CBA Trans-Activators biology.protein Cancer research Experimental pathology Female Tumor Suppressor Protein p53 |
| Zdroj: | Integrative Cancer Therapies. 10:168-177 |
| ISSN: | 1552-695X 1534-7354 |
| DOI: | 10.1177/1534735410380613 |
| Popis: | Unlike other forms of hepatocellular carcinoma (HCC), HCC induced by hepatitis B virus (HBV) infection shows a poor prognosis after conventional therapies. HBV induces liver cirrhosis and HCC. Many researchers have made efforts to find new substances that suppress the activity of HBV. Curcuma longa Linn (CLL) has been used for traditional medicine and food in Asia, especially in India, and has shown chemopreventive effects in a HBV-related in vitro model. This in vivo study was designed to seek the chemopreventive effects of CLL and its mechanisms. CLL mixture concentrated with dextrose water by boiling was lyophilized. CLL extracts were administrated to HBV X protein (HBx) transgenic mice aged 4 weeks for 2 to 4 weeks and aged 6 months for 3 months. After administration, histological changes in the liver tissue and expression of HBx-related genes were investigated. CLL-treated mice showed less visceral fat, a smaller liver/body weight ratio and delayed liver pathogenesis. Proliferating cell nuclear antigen (PCNA) expression was also increased in CLL-treated HBx transgenic mice, indicating regeneration of damaged liver tissue. CLL treatment decreased expression of HBx and increased p21 and cyclin D1 in livers of HBx transgenic mice. In addition, p-p53 was increased after CLL treatment. These results suggest that CLL can have beneficial effects on the early and late stages of liver pathogenesis, preventing and delaying liver carcinogenesis. This drug should be considered as a potential chemopreventive agent for HBV-related hepatocarcinogenesis. |
| Databáze: | OpenAIRE |
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