Uptake of dolichol by Vero cells
| Autor: | Albert R. Lagrou, Van Meirvenne H, W. Dierick, Herwig Hilderson, Van Dessel G |
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| Rok vydání: | 1992 |
| Předmět: |
Time Factors
Acyclic Monoterpenes Lipoproteins Kinetics Biology Biochemistry chemistry.chemical_compound Dolichol Dolichols Chlorocebus aethiops Animals Vitamin A Vero Cells Molecular Biology Terpenes Temperature Biological Transport Cell Biology Hydrogen-Ion Concentration Farnesol Cholesterol chemistry Liposomes Vero cell lipids (amino acids peptides and proteins) Subcellular Fractions |
| Zdroj: | Biochemistry and Cell Biology. 70:475-480 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o92-073 |
| Popis: | Characterization and kinetics of dolichol uptake by a Vero cell line are reported. Vero cells incorporate dolichol in a time- and dose-dependent manner. Optimal uptake is found at 37 °C and at a pH of 7.4. In contrast to cholesterol, an inhibitory effect on the dolichol incorporation is found for farnesol, geraniol, and retinol. Long chain polyprenols were slightly stimulatory. The translocation seems not to be highly energy dependent. The lack of substantial inhibition by chloroquine does not plead for a receptor-mediated endocytosis. Incorporated dolichol was distributed over both membranes and supernatant fractions, paralleling the distribution of the lysosomal marker β-N-acetylhexosaminidase. The incorporated dolichol is subject to a fast efflux process, which is potentiated by the presence of lipid acceptors in the extracellular medium.Key words: dolichol, efflux, subcellular fractionation, uptake, Vero cells. |
| Databáze: | OpenAIRE |
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