Renal tubule Na,K-ATPase polarity in different animal models of polycystic kidney disease
| Autor: | K. Tomobe, J. F. S. Crocker, S. Sareen, Hisahide Takahashi, Malcolm R. Ogborn |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Histology Rats Sprague-Dawley Mice medicine Polycystic kidney disease Animals Cyst Na+/K+-ATPase chemistry.chemical_classification Mice Inbred C3H Polycystic Kidney Diseases biology medicine.disease Molecular biology In vitro Rats Mice Inbred C57BL Disease Models Animal Kidney Tubules Enzyme chemistry Mice Inbred DBA Polyclonal antibodies biology.protein Immunohistochemistry Rabbits Sodium-Potassium-Exchanging ATPase Anatomy Antibody |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 43:785-790 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/43.8.7622841 |
| Popis: | Apical mislocation of the ubiquitous transport enzyme Na,K-ATPase has been implicated as a feature of cyst development in in vitro studies of human polycystic kidney disease (PKD) epithelia. We undertook an immunohistochemical study of murine glucocorticoid-induced PKD, the pcy mouse, the cpk mouse, and the diphenylthiazole (DPT)-induced rat models of PKD to determine if this feature was common to these models of cyst development. Distribution of Na,K-ATPase was determined with a polyclonal anti-Na,K-ATPase antibody and a nickel-silver-enhanced peroxidase color development system. Results were documented objectively with densitometric techniques. Control animals appropriate to the age, strain, and species of the experimental groups demonstrated the expected polar distribution of Na,K-ATPase to the basolateral surface. This distribution was more marked in mature animals. Tubular dilatation and cystic change, however, were associated with increased apical Na,K-ATPase in all models. The murine models demonstrated decreased basolateral staining for Na,K-ATPase compared with controls, although this was not a feature of the DPT rat model. Abnormal location of Na,K-ATPase is a shared feature of a variety of animal models and human PKD. This may contribute to abnormal fluid and electrolyte flux favoring cyst formation or may represent expression of a less differentiated renal tubule epithelial phenotype. |
| Databáze: | OpenAIRE |
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