An IL-15 Dependent CD8 T Cell Response to Selected HIV Epitopes is Related to Viral Control in Early-Treated HIV-Infected Subjects
| Autor: | Valerio Tozzi, Gianpiero D'Offizi, Filippo Martini, Roberto Paganelli, Alessandra Sacchi, Cristiana Gioia, Fabrizio Poccia, Angela Corpolongo, Pasquale Narciso, Ilaria Volpi |
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| Přispěvatelé: | D'Offizi, G., Gioia, C., Corpolongo, A., Martini, F., Paganelli, R., Volpi, I., Sacchi, A., Tozzi, V., Narciso, P., Poccia, F. |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Anti-HIV Agents HIV Antigens medicine.medical_treatment Immunology HIV Infections CD8-Positive T-Lymphocytes Virus Replication gag Gene Products Human Immunodeficiency Virus Epitope Flow cytometry Epitopes Interferon-gamma 03 medical and health sciences 0302 clinical medicine Antiretroviral Therapy Highly Active Humans Immunology and Allergy Medicine Cytotoxic T cell nef Gene Products Human Immunodeficiency Virus Cytokine Interleukin-15 Pharmacology Immunodominant epitope medicine.diagnostic_test business.industry ELISPOT Maturative pattern Drug holiday Middle Aged Virology Peptide Fragments Recombinant Proteins In vitro Interleukin 15 030220 oncology & carcinogenesis HIV-1 Leukocytes Mononuclear STI business Immunologic Memory CD8 T lymphocyte 030215 immunology |
| Zdroj: | International Journal of Immunopathology and Pharmacology. 20:473-485 |
| ISSN: | 2058-7384 |
| DOI: | 10.1177/039463200702000306 |
| Popis: | In some early-treated HIV+ patients, Structured Treatment Interruption (STI) is associated to spontaneous control of viral rebound. Thus, in this clinical setting, we analyzed the immunological parameters associated to viral control. Two groups of early treated patients who underwent STI were retrospectively defined, according to the ability to spontaneously control HIV replication (Controller and Non-controller). Plasma cytokine levels were analyzed by multiplex analysis. CD8 T cell differentiation was determined by polychromatic flow cytometry. Antigen-specific IFN-Γ production was analyzed by ELISpot and intracellular staining after stimulation with HIV-peptides. Long-term Elispot assays were performed in the presence or absence of IL-15. Plasma IL-15 was found decreased over a period of time in Non-Controller patients, whereas a restricted response to Gag (aa.167–202 and 265–279) and Nef (aa.86–100 and 111–138) immunodominant epitopes was more frequently observed in Controller patients. Interestingly, in two Non-Controller patients the CD8-mediated T cells response to immunodominant epitopes could be restored in vitro by IL-15, suggesting a major role of cytokine homeostasis on the generation of protective immunity. In early-treated HIV+ patients undergoing STI, HIV replication control was associated to CD8 T cell maturation and sustained IL-15 levels, leading to HIV-specific CD8 T cell responses against selected Gag and Nef epitopes. |
| Databáze: | OpenAIRE |
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