Efficacy of orally administered porcine epidemic diarrhea vaccine-loaded hydroxypropyl methylcellulose phthalate microspheres and RANKL-secreting L. lactis
Autor: | Sang-Kee Kang, Dong-Jun An, Bang-Hun Hyun, Da-Chuan Piao, Bong-Kyun Park, Ra Mi Cha, Jihye Shin, SeEun Choe, Yun-Jaie Choi, Sok Song, Gyu-Nam Park |
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Rok vydání: | 2020 |
Předmět: |
HPMCP
Swine animal diseases Administration Oral Methylcellulose Antibodies Viral Microbiology Article Viral Proteins 03 medical and health sciences Antigen Pregnancy Oral vaccine Animals Neutralizing antibody 030304 developmental biology Swine Diseases 0303 health sciences General Veterinary biology 030306 microbiology Colostrum Porcine epidemic diarrhea virus Viral Vaccine RANK Ligand PEDV food and beverages Viral Vaccines General Medicine biology.organism_classification Antibodies Neutralizing Virology Microspheres Vaccination stomatognathic diseases Lactobacillus Titer Vaccines Subunit biology.protein Soluble aP2 Female Antibody Coronavirus Infections |
Zdroj: | Veterinary Microbiology |
ISSN: | 0378-1135 |
Popis: | Highlights • HPMCP (aP2) plus LL RANKL is an oral PED vaccine for pregnant pigs. • The oral vaccine increases IgA antibody titers in colostrums significantly. • The oral vaccine yields survival rates similar to those of a commercial killed vaccine. Here, we examined the efficacy of are combinant subunit antigen-based oral vaccine for preventing porcine epidemic diarrhea virus (PEDV). First, we generated a soluble recombinant partial spike S1 protein (aP2) from PEDV in E. coli and then evaluated the utility of aP2 subunit vaccine-loaded hydroxypropyl methylcellulose phthalate microspheres (HPMCP) and RANKL-secreting L. lactis (LLRANKL) as a candidate oral vaccine in pregnant sows. Pregnant sows were vaccinated twice (with a 2 week interval between doses) at 4 weeks before farrowing. Titers of virus-specific IgA antibodies in colostrum, and neutralizing antibodies in serum, of sows vaccinated with HPMCP (aP2) plus LL RANKL increased significantly at 4 weeks post-first vaccination. Furthermore, the survival rate of newborn suckling piglets delivered by sows vaccinated with HPMCP (aP2) plus LL RANKL was similar to that of piglets delivered by sows vaccinated with a commercial killed porcine epidemic diarrhea virus (PED) vaccine. The South Korean government promotes a PED vaccine program (live-killed-killed) to increase the titers of IgA and IgG antibodies in pregnant sows and prevent PEDV. The oral vaccine strategy described herein, which is based on a safe and efficient recombinant subunit antigen, is an alternative PED vaccination strategy that could replace the traditional strategy, which relies on attenuated live oral vaccines or artificial infection with virulent PEDV. |
Databáze: | OpenAIRE |
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