Cardioprotective effects of 70-kDa heat shock protein in transgenic mice
| Autor: | Kathy H. Graves, R. S. Williams, A. D. Sherry, Craig R. Malloy, Ivor J. Benjamin, Piyu Zhao, Maggy Fina, Sandhya Gavva, Nina B. Radford, Randall W. Moreadith, A. J. Wiethoff |
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| Rok vydání: | 1996 |
| Předmět: |
Genetically modified mouse
High-energy phosphate Magnetic Resonance Spectroscopy Multidisciplinary Adenine Nucleotides Myocardium Transgene Ischemia Mice Transgenic Metabolic acidosis Biology medicine.disease Cell biology Mice Biochemistry Adenine nucleotide Reperfusion Injury Heat shock protein medicine Animals Humans HSP70 Heat-Shock Proteins Energy Metabolism Reperfusion injury Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:2339-2342 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70-kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart. |
| Databáze: | OpenAIRE |
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