Genetic Diversity and Host Alternation of the Egg Parasitoid Ooencyrtus pityocampae between the Pine Processionary Moth and the Caper Bug
| Autor: | Murad Ghanim, Zvi Mendel, Alex Protasov, Manuela Branco, Shahar Samra |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Molecular Sequence Data
Wasps Population lcsh:Medicine Population genetics Moths caper bug Generalist and specialist species Host-Parasite Interactions Parasitoid Electron Transport Complex IV Heteroptera Species Specificity DNA Ribosomal Spacer Genetic variation Animals Sex Ratio host alternation Amplified Fragment Length Polymorphism Analysis lcsh:Science education Ooencyrtus pityiocampae DNA Primers Ovum Genetic diversity education.field_of_study Multidisciplinary Base Sequence biology Mediterranean Region Ecology Host (biology) lcsh:R fungi Genetic Variation genetic diversity Sequence Analysis DNA pine processionary moth biology.organism_classification Genetics Population Genetic structure lcsh:Q Seasons Research Article |
| Zdroj: | PLoS ONE Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP PLoS ONE, Vol 10, Iss 4, p e0122788 (2015) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0122788 |
| Popis: | Research Article The increased use of molecular tools for species identification in recent decades revealed that each of many apparently generalist parasitoids are actually a complex of morphologically similar congeners, most of which have a rather narrow host range. Ooencyrtus pityocampae (OP), an important egg parasitoid of the pine processionary moth (PPM), is considered a generalist parasitoid. OP emerges from PPM eggs after winter hibernation, mainly in spring and early summer, long before the eggs of the next PPM generation occurs. The occurrence of OP in eggs of the variegated caper bug (CB) Stenozygum coloratum in spring and summer suggests that OP populations alternate seasonally between PPM and CB. However, the identity of OP population on CB eggs seemed uncertain; unlike OP-PPM populations, the former displayed apparently high male/female ratios and lack of attraction to the PPM sex pheromone. We studied the molecular identities of the two populations since the morphological identification of the genus Ooencyrtus, and OP in particular, is difficult. Sequencing of COI and ITS2 DNA fragments and AFLP analysis of individuals from both hosts revealed no apparent differences between the OP-PPM and the OP-CB populations for both the Israeli and the Turkish OPs, which therefore supported the possibility of host alternation. Sequencing data extended our knowledge of the genetic structure of OP populations in the Mediterranean area, and revealed clear separation between East and West Mediterranean populations. The overall level of genetic diversity was rather small, with the Israeli population much less diverse than all others; possible explanations for this finding are discussed. The findings support the possibility of utilizing the CB and other hosts for enhancing biological control of the PPM info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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