Properties of a highly purified antifertility factor from human seminal plasma
| Autor: | T. K. Audhya, Lourens J.D. Zaneveld, J. C. Goodpasture, J. M. Reddy |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
endocrine system Hot Temperature Acrosome reaction Guinea Pigs Hyaluronoglucosaminidase Semen Fertilization in Vitro Biology In Vitro Techniques Andrology Mice Human fertilization Capacitation medicine Animals Humans Zona pellucida Acrosin urogenital system Cell Biology General Medicine Oocyte medicine.anatomical_structure Fertility Reproductive Medicine Concanavalin A Immunology biology.protein Sperm Motility Acrosome Sperm Capacitation |
| Zdroj: | Biology of reproduction. 27(5) |
| ISSN: | 0006-3363 |
| Popis: | A high molecular weight antifertility factor (AF-1) was obtained in a high degree of purity from human seminal plasma by ultracentrifugation, CM-cellulose and concanavalin A chromatography, and Sepharose 6B gel filtration. A final purification step involving preparative disc electrophoresis was occasionally required. AF-1 showed a dose-dependent inhibition of the in vitro fertilizing ability of capacitated mouse spermatozoa, causing 50% inhibition of fertilization at approximately 27.5 micrograms/10(6) spermatozoa. Removal of the follicle cell layer of the oocyte did not decrease the antifertility effect of AF-1 but inhibition of fertilization was no longer observed after dispersal of the zona pellucida. The effect of AF-1 was on the spermatozoa and not on the oocytes. These results show that AF-1 treatment prevents capacitated spermatozoa from penetrating the zona pellucida and possibly the follicle cell layer. The mechanism by which AF-1 does so is not known because AF-1 did not prevent the in vitro acrosome reaction of guinea pig spermatozoa, nor did it inhibit the activity of human acrosin or bovine testicular hyaluronidase. The antifertility activity of AF-1 is reversed after recapacitation of the AF-1 treated spermatozoa and it can be assumed that AF-1 is either dissipated or loses activity during the transport of the spermatozoa through the female genital tract when capacitation takes place. AF-1 is heat labile. The properties of AF-1 are the same as those found previously for the pellet obtained by high-speed centrifugation of human seminal plasma, indicating that this is the primary, nonparticulate, high molecular weight factor with antifertility activity in human semen. |
| Databáze: | OpenAIRE |
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