Detection Methods Fit-for-Purpose in Enforcement Control of Genetically Modified Plants Produced with Novel Genomic Techniques (NGTs)
| Autor: | Frank Narendja, Walter Stepanek, Alexandra Ribarits, Rupert Hochegger |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Traceability GMO Computer science fungi 010401 analytical chemistry Sequencing data lcsh:S regulation enforcement Genetically modified crops Computational biology 01 natural sciences Genome 0104 chemical sciences lcsh:Agriculture Targeted Modification 03 medical and health sciences Genome editing genome editing Digital polymerase chain reaction detection methods Enforcement Agronomy and Crop Science 030304 developmental biology |
| Zdroj: | Agronomy, Vol 11, Iss 61, p 61 (2021) |
| ISSN: | 2073-4395 |
| Popis: | The comprehensive EU regulatory framework regarding GMOs aims at preventing damage to human and animal health and the environment, and foresees labelling and traceability. Genome-edited plants and products fall under these EU GMO regulations, which have to be implemented in enforcement control activities. GMO detection methods currently used by enforcement laboratories are based on real-time PCR, where specificity and sensitivity are important performance parameters. Genome editing allows the targeted modification of nucleotide sequences in organisms, including plants, and often produces single nucleotide variants (SNVs), which are the most challenging class of genome edits to detect. The test method must therefore meet advanced requirements regarding specificity, which can be increased by modifying a PCR method. Digital PCR systems achieve a very high sensitivity and have advantages in quantitative measurement. Sequencing methods may also be used to detect DNA modifications caused by genome editing. Whereas most PCR methods can be carried out in an enforcement laboratory with existing technical equipment and staff, the processing of the sequencing data requires additional resources and the appropriate bioinformatic expertise. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|