Comparison of nucleostemin gene expression in CD133+ and CD133- cell population in colon cancer cell line HT29
| Autor: | Noosha Zia-Jahromi, Kazem Parivar, Seyed Hossein Hejazi, Mojtaba Panjepour, Marjan Gharagozloo |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Colorectal cancer
medicine.drug_class Cell Population Gene Expression Biology Monoclonal antibody lcsh:RC254-282 Flow cytometry Immunophenotyping Antigens CD GTP-Binding Proteins Gene expression medicine Humans Radiology Nuclear Medicine and imaging CD133 AC133 Antigen education neoplasms Glycoproteins education.field_of_study medicine.diagnostic_test Nuclear Proteins General Medicine medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology digestive system diseases HT29 carbohydrates (lipids) medicine.anatomical_structure Oncology colon cancer Monoclonal embryonic structures Colonic Neoplasms biology.protein biological phenomena cell phenomena and immunity Peptides Phycoerythrin nucleostemin HT29 Cells |
| Zdroj: | Journal of Cancer Research and Therapeutics, Vol 10, Iss 1, Pp 68-72 (2014) |
| ISSN: | 1998-4138 |
| Popis: | Background: Nucleostemin has been shown to be essential for proliferation and survival of colon cancer cells. In this study, we evaluate and comparing nucleostemin expression in CD133+ and CD133- colon cancer cell line HT29. Materials and Methods: After preparation and culturing of HT29 cell line, isolation was performed using magnetic cell separation system by CD133 MicroBeads and phycoerythrin conjugated to monoclonal anti-human CD133 monoclonal antibody and analyzed with flow cytometry. For quantitative expression of nucleostemin in HT29, CD133+ and CD133- cells used specific nucleostemin primer and glyceraldehyde 3-phosphate dehydrogenase primer as endogenous control. Results: The results showed the percentage of CD133+ cells in HT29 colon cancer cell line ranged from 36.5% to 41.5%, whereas the percentage of CD133- cell ranged from 58.5% to 63.5%. The expression rate of nucleostemin in HT29, CD133+ and CD133- cells were 1.44 ± 0.78, 1.60 ± 0.70 and 1.00 ± 0.18 (respectively). The comparison of expression rate represents no significant difference in nucleostemin expression in CD133+, CD133- and HT29 colon cancer cells. Conclusion: It is concluded that nucleostemin expression could not be specific in a certain type of cells in colon cancer cell line HT29 and controlling strategies in colon cancer must not be focused on one certain type of colon cancer cells as main expressing nucleostemin gene. |
| Databáze: | OpenAIRE |
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