Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration
| Autor: | Gilson Khang, Mohammad Amjad Hossain, Jong-Hoon Kim, Nam-Soo Kim, Ki-Chul Hwang, Judith Sharmila John Peter, Aravinthan Adithan, Muthukumar Thangavelu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Bone Regeneration
Ginsenosides Cell Survival Composite number Osteocalcin chemistry.chemical_element 02 engineering and technology engineering.material Calcium Biochemistry Apatite Collagen Type I Chitosan Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Mice X-Ray Diffraction Structural Biology Spectroscopy Fourier Transform Infrared Animals Humans Bone regeneration Molecular Biology 030304 developmental biology Ginseng Compound 0303 health sciences Mesenchymal Stem Cells General Medicine DNA 021001 nanoscience & nanotechnology Microspheres chemistry Chemical engineering Gene Expression Regulation visual_art visual_art.visual_art_medium engineering NIH 3T3 Cells Osteopontin Biopolymer Hydroxyapatites Biocomposite 0210 nano-technology Porosity |
| Zdroj: | International journal of biological macromolecules. 146 |
| ISSN: | 1879-0003 |
| Popis: | There is a substantial for the bone graft materials in the clinical field. Porous, stable and biodegradable bone microsphere scaffold using biopolymer chitosan was studied, and biphasic calcium phosphate was added to improve mechanical and osteoconductivity properties later ginseng compound K was added for improving its medicinal properties. They were characterized using FTIR and XRD that showed the apatite crystal in the composite microsphere scaffolds were structurally similar to that of biogenic apatite crystals. Scanning electron microscopy images confirmed the presence of hydroxyapatite on the surface of the composite microspheres. In vitro results infers that the composite microspheres are biocompatible with NIH 3T3 and MG63 cells and capable of supporting growth and spreading of MG-63 cells. Further, Osteogenic markers expression was found to be higher in rat bone marrow stem cells seeded on microsphere scaffolds compared to control. The prepared biocomposite porous microsphere scaffold developed in this study can be used as an alternative for the bone regeneration or bone tissue engineering. |
| Databáze: | OpenAIRE |
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