Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
Autor: | Naoki Sugimoto, Yuzo Nishizaki, Kenji Matsui, Saisamorn Lumyong, Jaturong Kumla, Jomkwan Meerak, Nakarin Suwannarach |
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Rok vydání: | 2019 |
Předmět: |
Cinnamomum zeylanicum
Nitrogen Genes Fungal Anthraquinones Fungus Applied Microbiology and Biotechnology DNA Ribosomal Plant use of endophytic fungi in defense 03 medical and health sciences Pigment Ascomycota Yeast extract Food science Internal transcribed spacer Coloring Agents Ribosomal DNA Phylogeny 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry Methanol Textiles Temperature General Medicine Pigments Biological Hydrogen-Ion Concentration biology.organism_classification Carbon Staining Culture Media Solubility visual_art visual_art.visual_art_medium Natural dye Biotechnology |
Zdroj: | Applied microbiology and biotechnology. 103(17) |
ISSN: | 1432-0614 |
Popis: | Some of the most important natural pigments have been produced from fungi and used for coloring in food, cosmetics, textiles, and pharmaceutical products. Forty-seven isolates of endophytic fungi were isolated from Cinnamomum zeylanicum in northern Thailand. Only one isolate, CMU-ZY2045, produced an extracellularly red pigment. This isolate was identified as Nigrospora aurantiaca based on morphological characteristics and the molecular phylogenetic analysis of a combined four loci (large subunit and internal transcribed spacer of ribosomal DNA, β-tubulin, and translation elongation factor 1-alpha genes). The optimum conditions for red pigment production from this fungus were investigated. The results indicated that the highest red pigment yield was observed in the liquid medium containing glucose as a carbon source and yeast extract as a nitrogen source, at a pH value of 5.0 and at 27 °C with shaking for 5 days. The crude red pigment revealed the highest level of solubility in methanol. A fungal red pigment was found to have high stability at temperatures ranging from 20 to 50 °C and pH values at a range of 5.0–6.0. Based on liquid chromatography-mass spectrometry analyses, the red pigment was characterized as bostrycin. The extracted pigment was used for the textile dyeing process. Crude fungal red pigment revealed the highest staining ability in cotton fabrics and displayed excellent fastness to washing, which showing negative cytotoxicity at the concentrations used to cell culture. This is the first report on bostrycin production from N. aurantiaca. |
Databáze: | OpenAIRE |
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