Exploring a new approach for regenerative medicine: Ti-doped polycrystalline diamond layers as bioactive platforms for osteoblast-like cells growth
| Autor: | Lia Emauela Vanzetti, Rocco Carcione, Erica Iacob, Emanuela Tamburri, Lorenzo Lunelli, Maria Letizia Terranova, Cristina Potrich, Victor Micheli, Sara Politi, Giancarlo Pepponi, Ruben Bartali |
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| Rok vydání: | 2021 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Materials science General Physics and Astronomy 02 engineering and technology engineering.material Settore CHIM/03 010402 general chemistry 01 natural sciences Bioscaffold HF-CVD diamond Ti-doped diamond Tissue engineering chemistry.chemical_compound symbols.namesake X-ray photoelectron spectroscopy hemic and lymphatic diseases Phase (matter) parasitic diseases Cell growth Doping Diamond Surfaces and Interfaces General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films body regions chemistry Chemical engineering engineering symbols Polystyrene 0210 nano-technology Raman spectroscopy |
| Zdroj: | Applied surface science 540 (2021). doi:10.1016/j.apsusc.2020.148334 info:cnr-pdr/source/autori:Carcione R.; Politi S.; Iacob E.; Potrich C.; Lunelli L.; Vanzetti L.E.; Bartali R.; Micheli V.; Pepponi G.; Terranova M.L.; Tamburri E./titolo:Exploring a new approach for regenerative medicine: Ti-doped polycrystalline diamond layers as bioactive platforms for osteoblast-like cells growth/doi:10.1016%2Fj.apsusc.2020.148334/rivista:Applied surface science/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:540 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2020.148334 |
| Popis: | This study explores the feasibility to use electroconductive Ti-doped polycrystalline diamond layers as scaffolds for tissue engineering. The synthesis of the diamond-based materials is accomplished in a HFCVD reactor where Ti(IV) acetyl acetonate powders are delivered by N2 fluxes to the growing diamond phase. In-depth investigations (Raman spectroscopy, SEM, AFM, XRD, XPS) allowed the characterization of the morphological/structural/compositional features and the properties of charge transport (KPFM, I-V) induced in the diamond layers by the incorporation of Ti-species. The bioactivity of the Ti-doped diamond surface was verified investigating the growth of MG-63 osteoblast-like cells by using MTT assays and confocal microscopy. The study evidenced a net increase of cell replication rate on diamond scaffolds after 4 days of incubation. After 6-days incubation, the cell growth on the Ti-doped diamond scaffolds increased up to 150% compared with the reference polystyrene tissue culture vessel, with a dominant presence of cells in active division. The cell behavior is discussed and related to the structural and functional surface properties of the Ti-diamond systems, acting as bioactive platforms able to offer an extremely beneficial environment for cell proliferation and viability. © 2020 Elsevier B.V. |
| Databáze: | OpenAIRE |
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