A study of noncultured extracted hair follicle outer root sheath cell suspension for transplantation in vitiligo
| Autor: | Aarti N Shah, Ritu K Marfatia, Siddhartha S Saikia |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
vitiligo
0301 basic medicine medicine.medical_specialty noncultured extracted hair follicle outer root sheath suspension Context (language use) Dermatology Vitiligo Melanocyte Biology Outer root sheath 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine medicine melanocytes stem cells Follicular unit extraction integumentary system Hair follicle medicine.disease Trypsinization Surgery Transplantation 030104 developmental biology medicine.anatomical_structure Original Article Follicular Unit Extraction |
| Zdroj: | International Journal of Trichology |
| ISSN: | 0974-7753 |
| DOI: | 10.4103/0974-7753.188042 |
| Popis: | Context: Vitiligo surgeries have come a long way from tissue grafts to cultured and non cultured cell transplantation. Extracted hair follicle outer root sheath cell transplantation (EHF ORS) suspension is more enriched with melanocyte. In a hair bulb, there is one melanocyte for every five keratinocytes which is much higher than the epidermal melanin unit. Aims: To analyse the effectiveness of cultured EHF ORS and to perform objective evaluation based on clinical improvement & photographic evidence. To observe any untoward events or side effects. Settings and Design: The study was open and uncontrolled. All the patients were screened at preliminary visit. Reviews were done every two weeks. The endpoint selected was six months post procedure. Materials and Methods: Twenty five patients of stable Vitiligo were included in the study and follicular unit were harvested by Follicular Unit Extraction method. Outer root sheath cells were extracted by trypsinization. The solution was transplanted over dermabraded recipient site. Pressure dressing was given. Patients were followed up regularly. Statistical Analysis Used: Descriptive Statistics, Chi-Square. Results: Mean ± SD repigmentation was 80.15% ± 22.9% with excellent repigmentation (90-100%) in 60% of patients. Conclusions: This method is safe, effective, and simpler than the other methods involving cell culturing and requiring a laboratory set-up but selection of patients is crucial for the success of the outcome. |
| Databáze: | OpenAIRE |
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